Expression and production of proteins containing in hemidesmosme/besement membrane at the interface between the implant-oral epithelium.

种植体-口腔上皮之间界面处的半桥粒/基膜中含有的蛋白质的表达和产生。

• 批准号: 13470385
• 负责人: TANAKA Teruo
• 金额: $ 5.89万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470385/
• 关键词: titanium; oral epithelial cells; cell attachment; hemidesmosome; basement menbrane; laminin-5; PI3-kinase; F-アクチン; IGF; インプラント; ラミニン5; 免疫細胞科学; 歯肉; 接合上皮; ラット; 細胞培養; 免疫細胞化学

Laminin-5(Ln-5), which is associated with epithelial cell adhesion and migration, is present in the internal and external basement laminas(IBL and EBL) of the junctional epithelium(JE), whereas the detailed localization of Ln-5 in the peri-implant epithelium(PIE) remains unclear. By immuno-light microscopy, the immunoreactive product of Ln-5 appeared as a band along the implant-PIE interface, while most of the upper portion of the interface did not express Ln-5. Ln-5 was expressed along the connective tissue interfaces with the PIE, peri-implant sulcular epithelium, and oral epithelium. Immuno-electron microscopy showed that Ln-5 was expressed in the cells of the innermost-third layer and basal layer of the PIE, and in the IBL and EBL of the PIE. It was characteristic that a 100-nm-wide Ln-5-positive IBL and hemidesmosomes(HDs) were formed only in the lower portion of the interface. In addition, Ln-5-positive EBL was formed at most PIE-connective tissue interface, although at some part … More s of the interface, EBL and HDs were not formed. Next, we investigated the distribution of Ln-5 during the formation of PIE. One day after extraction, the JE had disappeared. After 3 days, Ln-5-positive epithelial cells extended horizontally over the wound at the leading edge from the oral sulcular epithelium(OSE). After 5 days, the epithelium extending from the OSE on each side of the wound joined and formed additional new epithelium. The new epithelium expressed Ln-5 in the BM. After 1 to 2 weeks, the oral epithelium extending from the sides of the wound joined in the center. Three days after implantation, no JE remained. Ln-5-positive epithelial cells extended apically from the OSE. After 1 to 2 weeks, the PIE was formed. Ln-5 was expressed at the PIE-connective tissue interface. Finally, after 4 weeks, Ln-5 was expressed at the implant-PIE interface, and the PIE was non-keratinized epithelium. Our findings suggest that partial deficiency of the IBL,EBL,and HDs in the PIE is associated with the presence of Ln-5, which is required for the formation of these attachment apparatuses, and that the PIE is a poorly adherent, permeable epithelium because of the partial deficiency of the attachment apparatuses, as compared to the JE. Furthermore it is suggested that Ln-5 induces cell migration during PIE formation, and that PIE originates from OSE. Less

层粘连蛋白-5（Ln-5）与上皮细胞的粘附和迁移有关，存在于连接上皮（JE）的内外基底板（IBL和EBL）中，而Ln-5在种植体周围上皮（PIE）中的具体定位尚不清楚。免疫光镜下，Ln-5的免疫反应产物沿种植体- pie界面呈带状，而界面上部大部分不表达Ln-5。Ln-5沿着与PIE、种植体周围管状上皮和口腔上皮的结缔组织界面表达。免疫电镜显示，Ln-5在PIE的最内三层和基底层细胞，以及PIE的IBL和EBL细胞中表达。其特点是仅在界面下部形成100 nm宽的ln -5阳性IBL和半半酶体（hd）。此外，在pie -结缔组织的大部分界面上都形成了ln -5阳性的EBL，尽管在部分界面上没有形成EBL和hd。接下来，我们研究了Ln-5在PIE形成过程中的分布。提取后1天，乙脑消失。3天后，ln -5阳性上皮细胞从口腔黏膜上皮（OSE）向创面前缘水平延伸。5 d后，伤口两侧从OSE延伸出来的上皮结合并形成新的上皮。BM中新生上皮表达Ln-5。1 ~ 2周后，从创面两侧伸出的口腔上皮在创面中心接合。植入3天后，未见乙脑残留。ln -5阳性上皮细胞从OSE向顶端延伸。1 ~ 2周后，PIE形成。Ln-5在pie -结缔组织界面表达。最后，4周后，Ln-5在种植体-PIE界面表达，PIE为非角化上皮。我们的研究结果表明，PIE中IBL、EBL和hd的部分缺乏与Ln-5的存在有关，而Ln-5是形成这些附着装置所必需的，与乙脑相比，PIE是一种粘附性差、渗透性差的上皮，因为附着装置的部分缺乏。进一步表明，在PIE形成过程中，Ln-5诱导细胞迁移，而PIE起源于OSE。少

项目成果

Kajiwara H, Yamaza T, Yoshinari M, Goto T, Iyama S, Atsuta I, Kido MA, Tanaka T.: "The bispohosphonate pamidronate on the surface of titanium stimulates bone formation around tibial implants in rats"Biomaterials. In press.

Kajiwara H、Yamaza T、Yoshinari M、Goto T、Iyama S、Atsuta I、Kido MA、Tanaka T.：“钛表面上的双磷膦酸帕米膦酸盐刺激大鼠胫骨植入物周围的骨形成”生物材料。

Ikiru Atsuta, Takayoshi Yamaza, Masao Yoshinari, Satoya Mino, Tetsuya Goto, Mizuho A Kido, Yoshihiro Terada, Teruo Tanaka: "Changes in the Distribution of Laminin-5 during Peri-Implant Formation after Immediate Titanium Implantation in Rats"Biomaterials.

Ikiru Atsuta、Takayoshi Yamaza、Masao Yoshinari、Satoya Mino、Tetsuya Goto、Mizuho A Kido、Yoshihiro Terada、Teruo Tanaka：“大鼠即刻钛植入后植入物周围形成过程中 Laminin-5 分布的变化”生物材料。

田中 輝男: "ザ・クインテッセンスVol.21 no.1"クインテッセンス出版. 11 (2002)

田中辉雄：《精粹 Vol.21 no.1》精粹出版社 11 (2002)。

Ikiru Atsuta, Takayoshi Yamaza, Masao Yoshinari, Satoya Mino, Tetsuya Goto, Mizuho A Kido, Yoshihiro Terada, Teruo Tanaka: "Changes in the Distribution of Laminin-5 during Peri-Implant Epithelium Formation after Immediate Titanium Implantation in Rats."Bi

Ikiru Atsuta、Takayoshi Yamaza、Masao Yoshinari、Satoya Mino、Tetsuya Goto、Mizuho A Kido、Yoshihiro Terada、Teruo Tanaka：“大鼠即刻钛植入后种植体周围上皮形成期间层粘连蛋白 5 的分布变化。”Bi

Hiroshi Kajiwara, Takayoshi Yamaza, Masao Yoshinari, Tetsuya Goto, Shinji Iyama, Ikiru Atsuta, Mizuho A.Kido, Teruo Tanaka: "The bisphosphonate pamidronate on the surface of titanium stimulates bone formation around tibial implants in rats."Biomaterials.

Hiroshi Kajiwara、Takayoshi Yamaza、Masao Yoshinari、Tetsuya Goto、Shinji Iyama、Ikiru Atsuta、Mizuho A.Kido、Teruo Tanaka：“钛表面的双膦酸盐帕米膦酸盐刺激大鼠胫骨植入物周围的骨形成。”生物材料。