Expression and production of proteins containing in hemidesmosme/besement membrane at the interface between the implant-oral epithelium.

层粘连蛋白-5(Ln-5)与上皮细胞的黏附和迁移有关，存在于连接上皮(JE)的内外基底层(IBL和EBL)，而Ln-5在种植周上皮(PIE)中的详细定位尚不清楚。免疫光学显微镜下，Ln-5免疫反应产物沿种植体-PIE界面呈条带状，而界面上部大部分不表达Ln-5。LN-5沿PIE结缔组织界面、种植体周沟上皮和口腔上皮细胞表达。免疫电子显微镜显示，Ln-5表达于馅饼最内层第三层和基底层的细胞，以及馅饼的IBL和EBL。其特点是仅在界面下部形成宽100 nm的Ln-5阳性IBL和半桥粒(HD)。此外，Ln-5阳性的EBL在大多数饼状-结缔组织界面上形成，尽管在某些部分…没有形成更多的S的界面、EBL和HDS。接下来，我们研究了Ln-5在馅饼形成过程中的分布。一天后，乙脑就消失了。3天后，Ln-5阳性的上皮细胞从口腔沟上皮(OSE)向创面前缘水平延伸。5d后，创面两侧OSE向外延伸的上皮结合形成新的上皮。新生上皮在骨髓中表达Ln-5。1~2周后，从创面两侧延伸出来的口腔上皮与中央相连。植入后三天，无乙脑残留。Ln-5阳性的上皮细胞从OSE向顶端延伸。1到2周后，馅饼就形成了。LN-5在PIE-结缔组织界面表达。4周后，Ln-5在种植体-PIE界面有表达，PIE为非角化上皮。我们的发现表明，PIE中IBL、EBL和HDs的部分缺失与Ln-5的存在有关，Ln-5是形成这些附着装置所必需的，与JE相比，由于附着装置的部分缺失，PIE是一种粘附性差、通透性差的上皮。推测Ln-5在PIE形成过程中诱导细胞迁移，PIE起源于OSE。较少

Laminin-5(Ln-5), which is associated with epithelial cell adhesion and migration, is present in the internal and external basement laminas(IBL and EBL) of the junctional epithelium(JE), whereas the detailed localization of Ln-5 in the peri-implant epithelium(PIE) remains unclear. By immuno-light microscopy, the immunoreactive product of Ln-5 appeared as a band along the implant-PIE interface, while most of the upper portion of the interface did not express Ln-5. Ln-5 was expressed along the connective tissue interfaces with the PIE, peri-implant sulcular epithelium, and oral epithelium. Immuno-electron microscopy showed that Ln-5 was expressed in the cells of the innermost-third layer and basal layer of the PIE, and in the IBL and EBL of the PIE. It was characteristic that a 100-nm-wide Ln-5-positive IBL and hemidesmosomes(HDs) were formed only in the lower portion of the interface. In addition, Ln-5-positive EBL was formed at most PIE-connective tissue interface, although at some part … More s of the interface, EBL and HDs were not formed. Next, we investigated the distribution of Ln-5 during the formation of PIE. One day after extraction, the JE had disappeared. After 3 days, Ln-5-positive epithelial cells extended horizontally over the wound at the leading edge from the oral sulcular epithelium(OSE). After 5 days, the epithelium extending from the OSE on each side of the wound joined and formed additional new epithelium. The new epithelium expressed Ln-5 in the BM. After 1 to 2 weeks, the oral epithelium extending from the sides of the wound joined in the center. Three days after implantation, no JE remained. Ln-5-positive epithelial cells extended apically from the OSE. After 1 to 2 weeks, the PIE was formed. Ln-5 was expressed at the PIE-connective tissue interface. Finally, after 4 weeks, Ln-5 was expressed at the implant-PIE interface, and the PIE was non-keratinized epithelium. Our findings suggest that partial deficiency of the IBL,EBL,and HDs in the PIE is associated with the presence of Ln-5, which is required for the formation of these attachment apparatuses, and that the PIE is a poorly adherent, permeable epithelium because of the partial deficiency of the attachment apparatuses, as compared to the JE. Furthermore it is suggested that Ln-5 induces cell migration during PIE formation, and that PIE originates from OSE. Less