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Genetic study on interaction among carcinogenesis, proliferation and progression in oral epithelia and mesenchymal tissues

口腔上皮和间质组织致癌、增殖和进展之间相互作用的遗传学研究

基本信息

批准号：
13470436
负责人：
INOUE Shingo
金额：
$ 9.28万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470436/
关键词：
mdm2 p53 squamous cell carcinoma leukoplakia oral lichen planus

项目摘要

I.The expression of cancer related genes (p53,mdm2,p14) in oral squamous cell carcinomas and leukoplakia were examined immunohistochemically.1.The expression rate of p53 was 23.2%, that of mdm2 was 44.6%, and that of p14 was 56.0% in leukoplakia. Only p14 expression was correlated with cellular atypism, and as atypism was severe, the rate of expression was reduced. The expression rate of p53 was 40.8%, that of mdm2 was 46.9% and that of p14 was 22.9% in oral squamous cell carcinomas, and as differentiation was poor, the expression rate was reduced.2.The expression rate of mdm2(+)/p14(+) was 85% in normal gingiva, 37% in leukoplakia (carcinogenesis), 30% in leukoplakia(no carcinogenesis), and 19% in squamous cell carcinomas. As atypical cells increased, the simultaneous expression rate of mdm2 and p14 was reduced. In squamous cell carcinoma, as differentiation was poor, the simultaneous expression rate of mdm2 and p14 was reduced.3.The only mdm2 expression rate was 0% in normal gingiva, 12% in leukoplakia (carcinogenesis) and 27.8% in oral squamous cell carcinomas. The expression of mdm2 was correlated with malignancy.4.The expression rate of mdm2 in stroma was 25.8% (non carcinogenesis) and 48.0% (carcinogenesis) in leukoplakia. According to the TNM classification, the mdm2 expression rate of early tumor stage cases was higher than that of advanced tumor stage cases.The expression rate of mdm2 of stroma was 7.7% in normal gingiva and 85% in oral lichen planus.

一、采用免疫组化方法检测口腔鳞癌和白斑中癌相关基因（p53、mdm 2、p14）的表达：1.白斑中p53的表达率为23.2%，mdm 2的表达率为44.6%，p14的表达率为56.0%。只有p14的表达与细胞凋亡相关，且随着凋亡程度的加重，表达率降低。口腔鳞癌中p53、mdm 2和p14的表达率分别为40.8%、46.9%和22.9%，且随着分化程度的降低，表达率降低（致癌作用），30%在白斑（无致癌作用），19%在鳞状细胞癌。随着非典型细胞增多，mdm 2和p14同时表达率降低。在口腔鳞状细胞癌中，随着分化程度的降低，mdm 2和p14的同时表达率降低。3. mdm 2在正常牙龈组织中的表达率为0%，在白斑（癌变）组织中的表达率为12%，在口腔鳞状细胞癌组织中的表达率为27.8%。mdm 2在白斑间质中的表达率为25.8%（非癌变）和48.0%（癌变）。根据TNM分期，mdm 2在早期肿瘤组织中的表达率高于晚期肿瘤组织，正常牙龈组织中mdm 2在间质中的表达率为7.7%，口腔扁平苔藓组织中mdm 2在间质中的表达率为85%。