Roles of cytokines and trophic factors on the plastic changes of dendritic morphology

细胞因子和营养因子对树突形态塑性变化的作用

• 批准号: 13480253
• 负责人: SENBA Emiko
• 金额: $ 8.7万
• 依托单位: Wakayama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13480253/
• 关键词: DRG; galectin-1; FK506; stress; hippocampus; BDNF; 5-HT; TROY; DRG; 細胞内カルシウム; Galectin-1; 神経幹細胞; LIF

1.Effects of endogenous lectin and immunosuppressant FK506 on sensory neurons1)Galectin-1(Gal-1) is involved in a variety of functions, including neurite outgrowth. About 20% of DRG neurons showed intense Gal-1-immunoreactivity, and most of them were c-Ret-positive. Intrathecal administration of anti-rhGal-1 antibody significantly attenuated the mechanical hypersensitivity induced by peripheral nerve injury, suggesting that endogenous Gal-1 potentiates neuropathic pain.2)FK506 shows multiple actions on neurons, although precise mechanisms are obscure. We addressed if FK506 directly activates sensory neurons or not. Ca^<2+> on cultured rat DRG neurons revealed FK506-induced increase in intracellular Ca^<2+> in a subpopulation of small DRG neurons (3.1%). When DRGs from inflamed rats were used, the incidence increased to 7.4%. FK506 sensitive neurons also responded to capsaicin and bradykinin. In the nerve-skin preparation, FK506 enhanced neuronal discharges of single C-fibers that are r … More esponsive to heat and bradykinin, indicating that FK506 activates nociceptive C-fibers (submitted to a journal).2.Factors that regulate dendritic morphology of hippocampal neurons in chronically stressed animalsRepetitive restrain stress for 3 weeks was shown to induce dendritic atrophy of pyramical cells in the hippocampus. We investigated BDNF mRNA levels in the hippocampus, together with plasma glucocorliocid (CC) levels, GC receptors in the hippocampus/hypothalamus and 5-HT synthesis in the raphe nuclei, in these animals, and suggested that CC-and 5-HT-induced down regulation of BDNF synthesis in the hippocampus plays a pivotal role in the regulation of dendritic morphology (submitted to a journal).3.Roles of a new member of TNF receptor superfamily TROY on the development of nervous systemWe investigated the expression pattern of TROY mRNA in the developing mice brain. TROYmRNA was strongly expressed in the ventiicular zone and subventiicular zone, which contain neuronal and glial precursors during embryogenesis, indicating that TROY plays important roles in the development of nervous system. Less

1.内源性凝集素和免疫抑制剂FK 506对感觉神经元的影响1）半乳糖凝集素-1（Galectin-1，Gal-1）参与多种功能，包括神经突生长。约20%的背根节神经元呈强烈的Gal-1免疫反应性，其中大部分为c-Ret阳性。鞘内注射抗rhGal-1抗体可显著减轻周围神经损伤引起的机械性超敏反应，提示内源性Gal-1可增强神经病理性疼痛。2）FK 506对神经元有多种作用，但确切机制尚不清楚。我们讨论了FK 506是否直接激活感觉神经元。培养的大鼠DRG神经元的Ca^<2+>显示，FK 506可诱导DRG小神经元亚群细胞内Ca^<2+>升高（3.1%）。当使用来自发炎大鼠的DRG时，发生率增加至7.4%。FK 506敏感神经元对辣椒素和缓激肽也有反应。在神经-皮肤制备中，FK 506增强了单个C纤维的神经元放电，这些C纤维是神经元的神经元。 ...更多信息 对热和缓激肽有反应，表明FK 506激活伤害感受性C纤维（提交给期刊）。2.调节慢性应激动物海马神经元树突形态的因素3周的重复束缚应激显示诱导海马锥体细胞的树突萎缩。我们研究了海马中BDNF mRNA的水平，以及血浆糖皮质激素（CC）水平，海马/下丘脑中的GC受体和中缝核中的5-HT合成，在这些动物中，提示CC和5-HT诱导的海马BDNF合成下调在树突形态调控中起着关键作用3.肿瘤坏死因子受体超家族新成员TROY在神经系统发育中的作用我们研究了TROY mRNA在小鼠脑发育过程中的表达模式。在胚胎发育过程中，TROY mRNA在含有神经元和神经胶质前体细胞的血管膜区和血管膜下区强表达，表明TROY在神经系统发育中起重要作用。少

项目成果

Imbe H.et al.: "Galectin-1 is involved in the potentiation of neuropathic pain in the dorsal hom."Blain Research. 993. 72-83 (2003)

Imbe H.等人：“Galectin-1 参与增强背侧神经性疼痛。”Blain Research。

Imbe H., Okamoto K.et al.: "Galectin-1 is involved in the potentiation of neuropathic pain in the dorsal horn."Brain Research. 993. 72-83 (2003)

Imbe H.、Okamoto K.等人：“Galectin-1 参与增强背角神经性疼痛。”大脑研究。

Ueyama, T., Senba, E., et al.: "Emotional stress activates MAP kinase in the rat heart"Life Science. 69. 1927-1934 (2001)

Ueyama, T.、Senba, E. 等人：“情绪压力激活大鼠心脏中的 MAP 激酶”生命科学。

Nanbu-Wakao R, Senba E et al.: "Stimulation of 3T3-L1 Adipogenesis by Signal Transducer and Activator of Transcription 5"Molecular Endocrinology. 16. 1565-1576 (2002)

Nanbu-Wakao R、Senba E 等人：“信号转导器和转录激活剂 5 刺激 3T3-L1 脂肪生成”分子内分泌学。

Kashiba H.et al.: "Distribution and colocalization of NGF and GDNF family ligand receptor mRNAs in dorsal root an d nodose ganglion neurons of adult rats."Molecular Brain Research. 110. 52-62 (2003)

Kashiba H.等人：“成年大鼠背根和结状神经节神经元中 NGF 和 GDNF 家族配体受体 mRNA 的分布和共定位。”分子脑研究。