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Tissue reconstruction with the use of partially decalcified bone autograft

使用部分脱钙自体骨移植进行组织重建

基本信息

批准号：
13480290
负责人：
NAGAI Masahiro
金额：
$ 7.36万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13480290/
关键词：
Biomaterials Ligament Regeneration Biological materials Bone 靱帯

项目摘要

Bone-decalcifled bone-bone grafts were made from rat s and rabbit s long tubular bones, and the ligament reconstruction utilizing them were investigated. Retrieved bones were partially covered by parafilm and the whole bones were Immersed into decalcified solutions. After decalcification collagen grafts with suitable flexibility and reasonable strength were generated. In vivo implantation tests into the subcutaneous tissue of experimental animals clarified the very good blocompatibilities of the materials.Ligament reconstruction experiments were carried out. In the reconstruction of rat s collateral ligaments, very early fusion of the interface between the grafts and bones was observed and strong fixation of grafts was expected. The Internal cavity of the graft was invaded by the new blood vessel and fibroblasts, and the remodeling from graft to mature ligament tissue progressed. In the reconstruction of rabbits cruclate ligaments of the knee, the nearly same procedure were repeated in this case but rupture of grafts was observed in some cases. Excessive load was believed to be loaded onto the grafts and mechanical failure was induced. Hence we introduced cross-link into the collagen of graft with the use of aldehyde or epoxy-compound and made strengthen the grafts. The reconstructed ligaments with epoxy-compound cross-linked grafts had suitable flexibility and about half number of the ligaments survived longer than 12 weeks after surgery. Furthermore, if the grafts were utilized together with the bloabsorbable materials reinforcement, almost all of the grafts survived and we succeeded reconstruction of ligaments.

以大鼠和家兔长管骨为材料制备脱钙骨移植物，并对其重建韧带进行了研究。取出的骨部分被旁膜覆盖，整个骨浸入脱钙溶液中。脱钙后可获得柔韧性适宜、强度合理的胶原移植物。实验动物皮下组织的体内植入试验表明，材料具有良好的血管相容性。进行韧带重建实验。在大鼠侧副韧带重建中，观察到移植物与骨之间的界面很早就融合，并且预计移植物的牢固固定。新生血管和成纤维细胞侵入移植物内腔，移植物向成熟韧带组织的重构进展。在兔膝关节十字韧带重建中，本病例重复了几乎相同的程序，但在某些情况下观察到移植物破裂。过度的负荷被认为是加载到移植物上，并引起机械故障。为此，我们利用醛或环氧化合物在接枝胶原中引入交联剂，使接枝增强。环氧化合物交联重建的韧带具有良好的柔韧性，约半数的韧带术后存活时间超过12周。此外，如果将移植物与可吸血材料加固一起使用，几乎所有的移植物都存活，我们成功地重建了韧带。