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High-throughput three-dimensional micro imaging using WDM technolgies

使用 WDM 技术的高通量三维显微成像

基本信息

批准号：
13555114
负责人：
NARUSE Makoto
金额：
$ 7.62万
依托单位：
National Institute of Information and Communications Technology (2002-2003)The University of Tokyo (2001)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

This research intends to achieve high-bandwidth three-dimensional micro imaging by fusing the idea of wavelength-domain multiplexing of light-wave and two-dimensional space-domain parallelism. For realize high-throughput systems, we need various new sub-system technologies concerning the essential properties of two-dimensional parallel optical devices, diffractive optical elements, integrated circuits, and so forth. In particular, the parallel structure, which is indispensable to achieve high-throughput, imposes new requirements to optical system designs. For example, in a conventional microscope objective, off-axis beams from a laser cannot be imaged onto a proper imaging position due to severe distortion of the lens system where the off-axis beam probing is not supposed. Clearly, a system-level problem occurs due to the introduction of parallelism into imaging applications.To solve such problems, we developed active alignment for the purpose of parallel system stabilization, optimal lens design for parallel confocal imaging, and data processing by using computational sensor array which is directly adapted to a parallel imaging optics. The active alignment was experimentally demonstrated where 118 Hz environmental disturbance was successfully reduced. Also, by employing H-infinity theory, we also realized to integrate the dynamics of the controller and disturbance for the operation of the active alignment. For the parallel confocal imaging system was developed by reducing distortion and astigmatic aberrations, which supports up to 3,200 parallel channels if we assume 250um pitch for the confocal microscopy.

本研究旨在融合光波的波长域复用和二维空域并行的思想，实现高带宽的三维微成像。为了实现高通量系统，我们需要各种新的子系统技术，涉及二维并行光学器件、衍射光学元件、集成电路等的基本特性。尤其是实现高吞吐量不可缺少的并行结构，对光学系统设计提出了新的要求。例如，在传统的显微镜物镜中，由于透镜系统的严重失真，来自激光的离轴光束不能成像到适当的成像位置，其中不应该进行离轴光束探测。显然，将并行性引入成像应用中会出现系统级的问题，为了解决这些问题，我们开发了用于并行系统稳定的主动对准、用于并行共焦成像的最佳透镜设计，以及使用直接与并行成像光学相适配的计算传感器阵列来进行数据处理。实验证明，主动对准成功地抑制了118赫兹的环境干扰。同时，利用H-无穷大理论，实现了控制器的动态特性与扰动的综合，从而实现了主动对准的操作。由于平行共焦成像系统是通过减少失真和像散像差而开发的，如果我们假设共焦显微镜的间距为250微米，它可以支持多达3,200个平行通道。