Inhibitory effect of adrenomedullin on uterine contraction

肾上腺髓质素对子宫收缩的抑制作用

• 批准号: 13557009
• 负责人: YANAGITA Toshihiko
• 金额: $ 8.96万
• 依托单位: Miyazaki Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13557009/
• 关键词: adrenomedullin; myometrium; tocolysis; pregnant uterus; placenta; decidua; mRNA expression; premature labor

To clarify the role of adrenomedullin (AM) in the female reproductive system, we examined the effects of AM on periodic uterine contraction in nonpregnant and pregnant rats.1. In isolated uterine strips of nonpregnant rat, adrenomedullin (AM) inhibited spontaneous and bradykinin-induced uterine contraction, but did not change oxytocin- or prostaglandin F_<2α>-induced uterine contraction. The inhibitory effect of AM was blocked by AM [22-52].2. During pregnancy, levels of AM mRNA and protein were significantly increased in decidua of placenta. The myometrium isolated from placental site did not contract spontaneously, whereas the periodic and strong contraction was induced by addition of AM [22-52].These results imply that increased level of AM in decidua inhibits the uterine contraction of placental site, and it might contribute to the maintainance of pregnancy without interfering normal labor promoted by oxytocin or PGF2_<2α>.

为了阐明肾上腺髓质素(AM)在雌性生殖系统中的作用，我们研究了AM对未怀孕和怀孕大鼠周期性子宫收缩的影响。在非妊娠大鼠的离体子宫条上，肾上腺髓质素(AM)抑制自发的和缓激肽引起的子宫收缩，但不改变催产素或前列腺素F&2α&gt；引起的子宫收缩。AM的抑制作用可被AM阻断[22-52]。妊娠期间，胎盘蜕膜中AM基因和蛋白的表达水平显著升高。从胎盘部位分离的子宫肌层不会自发收缩，而加入AM[22-52]可引起周期性的强烈收缩。这些结果表明，蜕膜中AM水平的升高抑制了胎盘部位的子宫收缩，可能有助于维持妊娠，而不影响催产素或PGF_2、α和gt；

项目成果

Kobayashi, Yanagita et al.: "Pathophysiological function of adrenomedullin and proadrenomedullin N-terminal peptides in adrenal chromaffin cells"Hypertens. Res.. Suppl.. S71-S78 (2003)

Kobayashi、Yanagita 等人：“肾上腺髓质素和肾上腺髓质素原 N 末端肽在肾上腺嗜铬细胞中的病理生理功能”Hypertens。

Yanagita et al.: "Regulation of uterine contractility by adrenomedullin in pregnant rat uterus"Japanese Journal of Pharmacology. 88(Suppl I). 146 (2002)

Yanagita等人：“妊娠大鼠子宫中肾上腺髓质素对子宫收缩力的调节”日本药理学杂志。

Yanagita T, Kobayashi H, Yamamoto R. Kitamura K, Asada Y, Ueta Y, Uezono Y, Yokoo H, Eto T, and Wada A: "Regulation of uterine contractility by adrenomedullin."Japanese Journal of Pharmacology. 85 (Suppl. I). 27 (2001)

Yanagita T、Kobayashi H、Yamamoto R. Kitamura K、Asada Y、Ueta Y、Uezono Y、Yokoo H、Eto T 和 Wada A：“肾上腺髓质素调节子宫收缩力。”日本药理学杂志。

Yanagita et al.: "Regulation of uterine contractility by adrenomedullin"Japanese journal of Pharmacology. 85(Suppl I). 27 (2001)

Yanagita等人：“肾上腺髓质素对子宫收缩力的调节”日本药理学杂志。

Yanagita T, Kobayashi H, Yamamoto R, Kitamura K, Asada Y, Ueta Y, Uezono Y, Yokoo H, Eto T, and Wada A: "Regulation of uterine contractility by adrenomedullin in pregnant rat uterus."Japanese Journal of Pharmacology. 88 (Suppl. I). 146 (2002)

Yanagita T、Kobayashi H、Yamamoto R、Kitamura K、Asada Y、Ueta Y、Uezono Y、Yokoo H、Eto T 和 Wada A：“妊娠大鼠子宫中肾上腺髓质素对子宫收缩力的调节”。《日本药理学杂志》。