Molecular Targeting Therapy with Intelligent Drug Delivery System using Transporter

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We performed the research works for enhancement the therapeutic effects of anti-cancer agents with the selective transfection of transporter genes into cancer cells in tumor bearing bodies. We elongated survival days with the administrations of Cisplatin entrapped transfferin binding PEG liposome for cancer peritonitis of gastric cancers, and with Oxaliplatin entrapped transfferin binding PEG liposome for cancer peritonitis of pancreatic cancers. We also developed the gene delivery systems of transporter genes. Anionic polyethylene glycol(PEG-C) binding polyethyleneimine with JTS-1, an pH sensitive fusogenic peptide was showed the enhancement of expression efficiency of Luciferase gene(6 x 10E9 RLU/mg protein). In the analysis of DNA microarray comparing with oxaliplatin sensitive cancer cell lines and resistant cancer cell lines, Tublin specific chaperon and CBP/P300-Interacting Transactivator (CITED2) genes were enhanced in the resistant cell lines. PEPT1, OATP-C and OCTN1 genes were founded with the analysis of transporter genes. PEPT1 is related the absorption of βlactum antibiotics, inhibitor of angiotensin converting enzyme, vestatin. OATP-C is related with exflux of peptide of hepatocytes. OCTN-1 is related to movement of pH dependent cationic electorates.