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Bone regeneration therapy using HAp/Cot with MSC.

使用 HAp/Cot 和 MSC 进行骨再生治疗。

基本信息

批准号：
13557125
负责人：
SHINOMIYA Kenichi
金额：
$ 9.02万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

When we started this study, hydroxyapatite/collagen nanocomposite (HAp/Col), which is an artificial bone incorporated into bone remodeling processes, had been developed. And tissue engineering with human mesenchymal stern cells (MS Cs)had been developed for clinical use of bone reconstruction. The purpose of this study was to develop the bone regeneration system using HAp/Col combined with MSC.At the beginning of this study, there had been only a dense block of HAp/Col, and it was not suitable or using as scaffold of MSC. For this reason or others, we, tried to develop some forms of HAp/Col. Porous HAp/Col is the most desirable as a scaffold of MSC among varieties of the HAp/Col implants developed in this study. In addition, the porous body of HAp/Col has also more favorable characteristics as artificial bone than the other forms of HAp/Cot implants. The development of the porous HAp/Col is ongoing, aiming to be a commercialized product.During this study, we had to study bone formation ability of MSC/scaffold hybrids without HAp/Col because porous HAp/Col had been under development 'Porous β-TCP (commercial product) was selected as the scaffold of MSC. We confirmed the bone formation ability of human MSC, but there were significant differences of theability among donors. A clinical application model of bone regeneration with MSC was also tested using the lumbar posterolateral fusion (PLF) model of monkey. PLF is one of the most difficult model to obtain successful results because the formed bone bridging transverse processes is ectopic bone and it must have enough strength to bear mechanical stresses from various directions. The results we obtained from the monkeys' PLF using MSC/β-TCP hybrids were superior to the conventional method using autograft bone. It would indicate the possibility that the use of MSC/β-TCP hybrids in clinical application is superior to conventional autograft bone.

当我们开始这项研究时，我们已经开发出了羟基磷灰石/胶原纳米复合材料(HAP/Col)，这是一种结合到骨重建过程中的人工骨。利用人骨髓间充质干细胞(MS-Cs)进行组织工程学已被用于骨重建的临床应用。本研究的目的是建立HAP/Col复合MSCs的骨再生系统。在本研究开始时，HAP/Col只有一块致密的块状材料，不适合或不适合作为MSC的支架。由于这样或那样的原因，我们试图开发几种形式的HAP/Col，在本研究开发的各种HAP/Col植入物中，多孔HAP/Col是最理想的MSC支架。此外，与其他形式的HAP/CoT种植体相比，HAP/Col的多孔体也具有更好的人工骨特性。在本研究中，由于多孔羟基磷灰石/胶原一直在开发中，我们不得不研究不含羟基磷灰石/胶原的基质细胞/支架杂化材料的成骨能力。本研究选择商品化的多孔β-TcP作为支架材料。我们证实了人MSC的成骨能力，但不同供者的成骨能力存在显著差异。采用猴腰椎后外侧融合(PLF)模型，对MSC骨再生的临床应用模型进行了验证。PLF是最难获得成功结果的模型之一，因为形成的骨桥接横突是异位骨，它必须有足够的强度来承受来自各个方向的机械应力。用MSC/β-Tcp杂交物进行猕猴骨髓间充质干细胞移植的结果优于传统的自体骨移植方法。提示骨髓间充质干细胞/β-磷酸三钙杂化材料临床应用优于传统的自体移植骨的可能性。