Bone regeneration therapy using HAp/Cot with MSC.

当我们开始这项研究时，羟基磷灰石/胶原纳米复合材料（HAp/Col），这是一种结合骨重建过程的人工骨，已经开发出来。利用人骨髓间充质干细胞构建的组织工程骨已初步应用于临床。本研究的目的是建立HA p/Col复合MSC的骨再生体系。由于这个原因或其他原因，我们试图开发一些形式的HAp/Col。多孔HAp/Col是本研究开发的各种HAp/Col植入物中最理想的MSC支架。此外，HAp/Col多孔体作为人工骨也比其他形式的HAp/Cot植入物具有更有利的特性。多孔HA p/Col的开发正在进行中，旨在成为商业化产品。在本研究中，我们不得不研究MSC/支架杂交体的成骨能力，而没有HA p/Col，因为多孔HA p/Col已经在开发中。我们证实了人MSC的成骨能力，但不同供体间的成骨能力存在显著差异。利用猴腰椎后外侧融合（PLF）模型对MSC骨再生的临床应用模型进行了测试。PLF是最难获得成功结果的模型之一，因为形成的桥接横突的骨是异位骨，它必须具有足够的强度来承受来自各个方向的机械应力。MSC/β-TCP复合物移植猴PLF的结果上级传统的自体骨移植方法。这将表明MSC/β-TCP混合物在临床应用中上级传统自体骨移植的可能性。

When we started this study, hydroxyapatite/collagen nanocomposite (HAp/Col), which is an artificial bone incorporated into bone remodeling processes, had been developed. And tissue engineering with human mesenchymal stern cells (MS Cs)had been developed for clinical use of bone reconstruction. The purpose of this study was to develop the bone regeneration system using HAp/Col combined with MSC.At the beginning of this study, there had been only a dense block of HAp/Col, and it was not suitable or using as scaffold of MSC. For this reason or others, we, tried to develop some forms of HAp/Col. Porous HAp/Col is the most desirable as a scaffold of MSC among varieties of the HAp/Col implants developed in this study. In addition, the porous body of HAp/Col has also more favorable characteristics as artificial bone than the other forms of HAp/Cot implants. The development of the porous HAp/Col is ongoing, aiming to be a commercialized product.During this study, we had to study bone formation ability of MSC/scaffold hybrids without HAp/Col because porous HAp/Col had been under development 'Porous β-TCP (commercial product) was selected as the scaffold of MSC. We confirmed the bone formation ability of human MSC, but there were significant differences of theability among donors. A clinical application model of bone regeneration with MSC was also tested using the lumbar posterolateral fusion (PLF) model of monkey. PLF is one of the most difficult model to obtain successful results because the formed bone bridging transverse processes is ectopic bone and it must have enough strength to bear mechanical stresses from various directions. The results we obtained from the monkeys' PLF using MSC/β-TCP hybrids were superior to the conventional method using autograft bone. It would indicate the possibility that the use of MSC/β-TCP hybrids in clinical application is superior to conventional autograft bone.