Establishment of transplantation methods by using neural stem cells and peripheral nerve tissues for spinal cord repair

体外扩增的神经干细胞（NSCs）移植是一种潜在的强大工具，以修复受损的脊髓功能。成功的移植治疗的先决条件是确定优化的实验参数，可以促进最大的生存，广泛的迁移，并选择性分化的移植NSC人口在spinal cord.We评估的基本特征的神经干细胞样细胞从两个不同的供体来源，胚胎海马和脊髓，移植到新生儿脊髓后。成纤维细胞生长因子2（fibroblastgrowthfactor2，FGF2）的浓度可调控两种神经干细胞的增殖和分化表型。这两种神经干细胞样细胞可以在完整的新生儿脊髓的环境中存活，并在移植后不久表现出广泛的迁移行为。然而，定量分析显示，88%的海马细胞优先定位于神经元。 ...更多信息 附着于背侧白色物质。相反，只有64%的脊髓细胞位于背侧白色物质中。两种神经干细胞移植后均表现出向少突胶质细胞谱系的限制性表型。两种细胞类型的混合物的移植揭示了来自海马的神经干细胞样细胞的选择性存活。BrdU增殖实验显示，10%的海马细胞和7%的脊髓细胞的BrdU免疫反应阳性。移植后1周内，大部分前体细胞通过背侧白色物质迁移，但无增殖，提示可移植来源于海马的前体细胞。神经干细胞为未成熟的少突胶质细胞提供细胞来源，少突胶质细胞被认为是脊髓背侧白色物质中再生轴突的髓鞘形成和营养支持所必需的。将神经干细胞与周围神经联合作为支架材料，可有效促进损伤脊髓的轴突再生。少

Transplantation of in vitro-expanded neural stem cells (NSCs) is a potentially powerful tool to repair functions of the injured spinal cord. A prerequisite for the successful transplantation therapy is identification of optimized experimental parameters that can promote maximal survival, extensive migration, and selective differentiation of the transplanted NSC population in the spinal cord.We evaluated the basic characteristics of NSC-like cells from two different donor sources, the embryonic hippocampus and spinal cord, after transplantation into the neonatal spinal cord. Proliferation and differentiation phenotypes of both NSC-like cells can be controlled by the concentration of the fibrobiast growth factor 2 (FGF2) in vitro. Both NSC-like cells can survive within the environment of the intact neonatal spinal cord and showed extensive migratory behavior shortly after transplantation. However, quantitative analysis revealed that 88% of total hippocampal cells were preferentially loca … More lized to the dorsal white matter. In contrast, only 64% of total spinal cord cells were in the dorsal white matter. Both NSC-like cells showed restricted phenotype toward the oligodendroglial lineage after transplantation. Transplantation of the mixture of two cell types revealed selective survival of hippocampus-derived NSC-like cells. BrdU proliferation assay revealed that 10% of total hippocampal cells and 7% of total spinal cord cells were immunoreactive with BrdU. A majority of progenitor cells migrated through dorsal white matter without proliferation during the period of one week after transplantation.This study indicates the possibility of transplanting hippocampus-derived. NSCs to supply the cell source for immature oligodendrocytes, which are thought to be essential for both the myelination and trophic support of regenerating axons in the dorsal white matter of the spinal cord. A combination of hippocampus-derived NSC and peripheral nerves as scaffolds of the cells could be effective treatment to regenerate axons in injured spinal cord. Less