DEVELOPMENT AND CLINICAL APPLICATION OF VECTOR INJECTION DEVICE FOR GENE THERAPY OF SOLID TUMOR

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We have innovated a new device, for vector injection for gene therapy of solid tumor and applied for patent. In this study we actually constructed this machine and preliminary checked its safety, condition and adjusted optimal condition for actual injection. In our series prostate was regarded as a target organ.1)Experiment using free meat block to determine optimal injection conditionBy injecting a dye into free meat block, optimal condition of injection was analyzed. As a result, injection speed at 0.1ml/second was thought to be optimal.2)Improvement of the deviceFor the improvement of the each part, a simulation was repeated. Major improvement is a formation of foot switch so as the operator can handle the device with his eye kept of the ultrasound screen. We made injection on speed and volume changeable so as we can change the injection condition depending on the tumor volume.3)Actual injection to the patinetsWe have a prostate cancer gene therapy protocol for the patients of local recurrence after hormonal therapy without evidence of metastasis. This protocol is approved by IRE and government. A replication-deficient adenovirus (ADV) containing the herpes simplex virus thymidine kinase gene (HSV-tk) is injected directly into the prostate under ultrasound guidance, followed by intravenous administration of the prodrug ganciclovir (GCV) for 14 days.. Seven cases were injected using this device and injections wete performed precisely and consistently. It is concluded that this device is reliable and useful for vector injection and applicable for the other cancer.