The development of functional collagen material which has a property of controlled growth factor release, and its application for tissue regeneration therapy.

具有控制生长因子释放特性的功能性胶原材料的开发及其在组织再生治疗中的应用。

• 批准号: 13557166
• 负责人: TAKAHASHI Junzo
• 金额: $ 7.49万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13557166/
• 关键词: Functional material; Collagen; Hydroxyapatite; Biodegradable; Controlled release of protein; Bone regeneration; 機能性ペプチド; 血管新生; 徐放性; ゼラチン; 担体; 代謝性

We could realize a large numbers of new genes and proteins with their functions by the advance of the molecular biology and the developmental biology during the last decade, and the people's expectation is growing towards the clinical application of the regenerative medicine by using such obtained knowledge. Bone and tooth, especially among the numerous living tissues, is expected as a regenerativable tissue in near future, because the mechanism of bone formation has well studied and understood. However, we have had few concrete strategies to create new bone for the clinical application at this point. In this study, in order to create a new methodology of generating new bone for clinical application, we tried to make a new functional material with specific property "controlled release of growth factors" by using collagen and hydroxyapatite.It was confirmed that hydroxyapatite synthesized at different temperature showed different bioabsorbability, and protein adsorption. When we prepared hydroxyapatite particles adsorbed protein and investigated the protein release from this apatite in several biologic conditions, the protein release could be controlled by apatite dissolution. When we combined apatite particles in collagen gel and investigated the biodegradation of this collagen/apatite composite material, the degradation of this material is depended on the containing amount of apatite. Moreover, it was confirmed that the apatite was released from the composite material during its degradation period. These results suggest that this composite material has a property of controlled release of protein.

近十年来，随着分子生物学和发育生物学的发展，我们可以认识到大量新的基因和蛋白质及其功能，人们对利用这些知识进行再生医学临床应用的期望越来越高。骨和牙齿，特别是在众多的活体组织中，由于骨的形成机制已经被很好地研究和理解，有望在不久的将来成为可再生的组织。然而，在这一点上，我们几乎没有具体的策略来创造临床应用的新骨。在本研究中，我们试图利用胶原蛋白和羟基磷灰石制备一种具有“生长因子控释”特性的新型功能材料，为临床应用创造一种生成新骨的新方法。结果表明，不同温度下合成的羟基磷灰石具有不同的生物吸收率和蛋白质吸附性能。制备了吸附蛋白质的羟基磷灰石颗粒，研究了不同生物条件下羟基磷灰石对蛋白质的释放，发现磷灰石的溶解可以控制蛋白质的释放。当我们将磷灰石颗粒与胶原凝胶结合，研究胶原/磷灰石复合材料的生物降解时，该材料的降解取决于磷灰石的含量。此外，还证实了复合材料在降解过程中有磷灰石析出。这些结果表明，该复合材料具有控制蛋白质释放的特性。

项目成果

T.Matsumoto, J.Takahashi et al.: "Hydroxyapatite particles as a controlled release carrier of protein."Biomaterials. 25. 3807-3812 (2004)

T.Matsumoto、J.Takahashi 等人：“羟基磷灰石颗粒作为蛋白质的控释载体。”生物材料。

T.Matsumoto, M.Okazaki, J.Takahashi: "Biodegradation of carbonate apatite / collagen composite membrane and its controlled"Journal of Biomedical and Material Research. 60・4. 651-656 (2002)

T.Matsumoto、M.Okazaki、J.Takahashi：“碳酸盐磷灰石/胶原复合膜的生物降解及其控制”生物医学和材料研究杂志60・4（2002）。

T.Matsumoto, M.Inoue, J.Takahashi: "Surface-Modified Hydroxyapatite as Carrier for Drug Delivery System"Journal Dental Research. 80. 1116 (2001)

T.Matsumoto、M.Inoue、J.Takahashi：“表面改性羟基磷灰石作为药物输送系统的载体”牙科研究杂志。

T.Matsumoto, M.Okazaki, J.Takahashi: "Low crystalline hydroxyapatite as the controlled release carrier of basic protein"Journal Dental Research. 81. A191 (2002)

T.Matsumoto、M.Okazaki、J.Takahashi：“低结晶羟基磷灰石作为碱性蛋白的控释载体”牙科研究杂志。

Nakao H et al.: "Control of pore size in L-lactide/epsilon-caprolactone copolymer foams for tissue regeneration by the freeze-drying method"Dental Materials Journal. 22. 262-271 (2003)

Nakao H 等人：“通过冷冻干燥方法控制用于组织再生的 L-丙交酯/ε-己内酯共聚物泡沫中的孔径”《牙科材料杂志》。