Modification of functional peptide SVVYGLR and its application for bone tissue regeneration

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Angiogenesis is crucial for osteo-chondral ossification. Functional peptide SVVYGLR composed of 7 amino acids residues and found as high angiogenic activity is known as a fragment of osteopontin. Here, we hypothesized that SVVYGLR peptide have an important role for bone regeneration, and investigated its effect on osteoblast function. SVVYGLR showed significantly high effect on cell attachment, however, the peptide did not indicate cell proliferation and cell differentiation effect. SVVYGLR has a high angiogenic activity and should be useful for promoting wound healing followed by bone regeneration. Moreover. there are few reports, in which mention the method for delivering such a small molecular weight and size peptide with materials. So, we investigated the optimal modification method and porosity control method for biodegradable material (e.g. poly lactic acid, poly lactic acid/ε-caprolactone co-polymaer) for creating optimal SVVYGLR delivery material. The porosity of poly lactic acidε-caprolactone co-polymaer is controlled by freezing speed (this changes growing size of ice crystals), when the porous structure is fabricated by freeze drying method. The porous size created by this method varied in a range of 10-110 μm. Air plasma irradiation to the surface of these polymers increased the number of hydrophilic residues, and this method was useful for immobilizing peptide dissolved in water on polymer surface. In vivo evaluation of angiogenic effect of this polymer modified by SVVYGLR showed significantly increased the number of newly formed vessels. In this study, we developed new modification methods for applying functional peptide with biodegradable materials for promoting wound healing including bone regeneration.