Establishment of Simple Method for Screening of Teratogenic Steroids in Early Development of Vertebrates

脊椎动物早期发育致畸类固醇简易筛查方法的建立

• 批准号: 13558085
• 负责人: NISHINO Hideaki
• 金额: $ 3.14万
• 依托单位: Sapporo School of The Arts (2002)Tokyo Metropolitan College (2001)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13558085/
• 关键词: Sonic hedgehog; cholesterol; steroid; teratogen; development; ソニックヘッジホッグ; 植物アルカロイド

Antibodies prepared in this study showed positive reaction only when the antigen, recombinant sonic hedgehog protein, was processed to mature form having new carboxy-terminus by incubation with cholesterol. The other steroids can be screened using this antibodies whether they process sonic hedgehog protein or not, that is, perturb the normal process of early development of vertebrates or not.

本研究制备的抗体仅在抗原重组超音刺猬蛋白与胆固醇孵育后加工到具有新羧基末端的成熟形态时才有阳性反应。其他类固醇无论是否加工超音hedgehog蛋白，即是否干扰脊椎动物早期发育的正常过程，都可以通过该抗体进行筛选。