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Clarification of oligosaccharides for modulation of common mucosal immune system by using formation of artificial oligosaccharide clusters

通过形成人工寡糖簇来澄清用于调节常见粘膜免疫系统的寡糖

基本信息

批准号：
14580617
负责人：
KIYOHARA Hiroaki
金额：
$ 1.92万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

By using structure-specific carbohydrases, the active site was analyzed for expression of intestinal immune system modulating activity of the arabinogalactan isolated from rhizomes of Atractylodes lancea DC. It was strongly suggested that the glycocluster, which was constructed from galactosyl side chains consisting partly of β-D-(1→6)-galactose and the main chain of β-D-(1→3)-galactan, express the intestinal immune system modulating activity. After the oligosaccharide side chains, which were liberated from the β-D-(1→3,6)-galactan moiety of the active arabinogalactan by enzymic digestion, were purifed to anlyze the structure, and it was found that the oligosaccharide side chains having d.p.2-8 were the linear oligosaccharides consisting only of β-D-(1→6)-galactose. The present study also showed that the synthesis of glycocluster of these oligosaccharides by using the artificial core was impossible to perform because of the steric exclusion during the synthetic procedure. The other intestinal immune system modulating polysaccharides consisting of β-D-(1→3,6)-galactan as the active sites were isolated from some plant materials, and the new method was developed for comparing oligosaccharide side chains in the β-D-(1→3,6)-galactan chains of these active polysaccharides, by using multivariate analysis. From this analysis, 5 oligosaccharide chains among 50 kinds of the side chains were selected as the commonly-detected oligiosaccharide sequences in the active polysaccharides, and it was assumed that these 5 selected oligosaccharide might be contribute to expression of the intestinal immune system modulating activity.

通过使用结构特异性糖酶，分析了从苍术根茎中分离出的阿拉伯半乳聚糖的肠道免疫系统调节活性位点的表达。强烈表明，由部分由β-D-(1→6)-半乳糖组成的半乳糖基侧链和β-D-(1→3)-半乳聚糖主链构成的糖簇表达肠道免疫系统调节活性。通过酶消化从活性阿拉伯半乳聚糖的β-D-(1→3,6)-半乳聚糖部分中分离出寡糖侧链，纯化后进行结构分析，发现具有d.p.2-8的寡糖侧链是仅由β-D-(1→6)-半乳糖组成的线性寡糖。本研究还表明，由于合成过程中的空间排斥，使用人工核心合成这些寡糖的糖簇是不可能的。从一些植物材料中分离出另一种以β-D-(1→3,6)-半乳聚糖为活性位点的肠道免疫系统调节多糖，并通过多变量分析开发了比较这些活性多糖的β-D-(1→3,6)-半乳聚糖链中寡糖侧链的新方法。通过该分析，从50种侧链中选择了5条寡糖链作为活性多糖中常见的寡糖序列，并推测这5种选定的寡糖可能有助于肠道免疫系统调节活性的表达。