The glycan modulation mechanism of activity vitronectin that induces tissue regeneration and remodeling, or tissue fibrosis

活性玻连蛋白诱导组织再生和重塑或组织纤维化的聚糖调节机制

基本信息

  • 批准号:
    14580622
  • 负责人:
  • 金额:
    $ 2.37万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2002
  • 资助国家:
    日本
  • 起止时间:
    2002 至 2003
  • 项目状态:
    已结题

项目摘要

Vitronectin (VN) is a multifunctional glycoprotein involved in tissue remodeling. Previously, we reported that glycosylation of VN significantly changes and enhances the collagen-binding activity^1). In this study, the effect on tissue lytic system and the cell-spreading activity of VN was focused.Rat VNs were purified after urea-denaturation form plasma 24 hours after partial hepatectomy (PH), sham-operation (SH) and non-operation (NO). The binding of PH-or SH-VN to stabilize plasminogen activator inhibitor-1 (PAI-1) decreased to 1/3 and 2/3, respectively, of that of NO-VN. The uPA-binding of both PH-and SH-VNs increased to 2 times of that of No-VN. Consequently the activites of uPA were increased in the presence of SH-or SH-VN compared with NO-VN. In contrast, deglycosylation of VNs by enzyme treatments enhanced both PAI-1 and uPA binding so that the resultant uPA acitivity was unchanged suggesting that the cach ligand-binding of VN is influenced complexly with the innerglycan structures.The cell spreading activity on VN substrate differed by the kind of cells The spreading of BHK on desialylated VN decreased to 1/2 of that of control VN, indicating that the cell-spreading activity is significantly affected by sialylation state of VN. However, on the VNs during liver regeneration, BHK cells exhibited slightly decreased spreading on SH-VN but similar spreading on PH-VN and NO-VN.PH-VN induced the enhanced uPA activity, which would promote plasmin generation. Therefore, the change in glycosylation of VN contributes to tissue remodeling by enhancing tissue lysis at the initial stage of liver regeneration.
玻连蛋白(VN)是一种多功能糖蛋白,参与组织重塑。之前,我们曾报道VN的糖基化显著改变并增强了胶原结合活性^[1]。本研究以大鼠肝部分切除(PH)、假手术(SH)和非手术(NO)后24 h血浆为材料,采用尿素变性法纯化VN,观察VN对组织溶解系统和细胞铺展活性的影响。PH-或SH-VN与纤溶酶原激活物抑制剂-1(派-1)的结合分别为NO-VN的1/3和2/3。PH-VN和SH-VN的uPA结合率均为No-VN的2倍。结果表明,与NO-VN相比,SH-VN或SH-VN可使uPA活性升高。相反,通过酶处理的VN的去糖基化增强了派-1和uPA结合,因此所得的uPA活性不变,这表明VN的cach配体结合受到内聚糖结构的复杂影响。表明VN的唾液酸化状态显著影响细胞铺展活性。在肝再生过程中,BHK细胞在VN上的铺展能力稍有下降,而在PH-VN和NO-VN上的铺展能力相似,PH-VN诱导uPA活性增强,这将促进纤溶酶的生成。因此,VN糖基化的变化有助于通过在肝再生的初始阶段增强组织溶解来促进组织重塑。

项目成果

期刊论文数量(74)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Ogawa, H., Ueda, H., Natsume, A., Suzuki, R.: "Preparation and utility of neoproteoglycan probes in the interaction a nalyses with glycosaminoglycan-binding proteins."Methods in Enzymology. 362. 196-209 (2003)
Okawa, H.、Ueda, H.、Natsume, A.、Suzuki, R.:“新蛋白聚糖探针在与糖胺聚糖结合蛋白相互作用分析中的制备和应用。”酶学方法。
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小川温子, 天野麻穂, 土方亜子, 加藤真理, 上平知子, 末次勧, 石塚稲夫: "花粉症アレルギーと糖鎖抗原"J.Appl.Glycosci.. 50. 327-331 (2003)
Atsuko Okawa、Maho Amano、Ako Hijikata、Mari Kato、Tomoko Kamihira、Kan Suetsugu、Inao Ishizuka:“花粉症过敏和碳水化合物抗原” J.Appl.Glycosci.. 50. 327-331 (2003)
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小川温子, 岩城はるひ(分担執筆): "血小板生物学(池田康夫, 丸山征郎編)"メディカルレビュー社(株)(印刷中). (2004)
小川敦子、岩城春日(撰稿人):《血小板生物学(池田康夫、丸山清朗编辑)》Medical Review Co., Ltd.(正在印刷)(2004 年)。
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    0
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Matsushita, H., Takenaka, M., Ogawa, H.: "Porcine pancreatic -amylase shows binding activity toward N-linked oligosaccharides of glycoproteins"J.Biol.Chem.. 277. 4680-4686 (2002)
Matsushita, H.、Takenaka, M.、Okawa, H.:“猪胰淀粉酶显示出对糖蛋白的 N-连接寡糖的结合活性”J.Biol.Chem.. 277. 4680-4686 (2002)
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    0
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小川温子, 天野麻穂, 土方亜子, 加藤真理, 上平知子, 末次勸, 石塚稲夫: "花粉症アレルギーと糖鎖抗原"J.Apple.Glycosci.. 50. 327-331 (2003)
Atsuko Okawa、Maho Amano、Ako Hijikata、Mari Kato、Tomoko Kamihira、Kan Suetsugu、Inao Ishizuka:“花粉症过敏和碳水化合物抗原” J.Apple.Glycosci.. 50. 327-331 (2003)
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OGAWA Haruko其他文献

OGAWA Haruko的其他文献

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{{ truncateString('OGAWA Haruko', 18)}}的其他基金

The significance of the carbohydrate recognition of trypsinogen in pancreatic exocrine mechanism
胰蛋白酶原碳水化合物识别在胰腺外分泌机制中的意义
  • 批准号:
    25440016
  • 财政年份:
    2013
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Regulation mechanism of nutrient assimilation and exocrine system which is achieved by the carbohydrate-recognition of pancreatic enzymes
胰酶识别碳水化合物实现营养同化和外分泌系统的调节机制
  • 批准号:
    22570111
  • 财政年份:
    2010
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of tolerance induction method using clone pigs remodeled for expressing human ABO blood group antigen
开发表达人ABO血型抗原的克隆猪耐受诱导方法
  • 批准号:
    21380168
  • 财政年份:
    2009
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
The generation of clone pigs expressing blood group antigens -developing a tolerance induction method-
表达血型抗原的克隆猪的产生-开发耐受性诱导方法-
  • 批准号:
    18380162
  • 财政年份:
    2006
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
The biological significance of the carbohydrate-binding activities found for pancreatic enzymes in secretion and digestion
胰酶在分泌和消化中发现的碳水化合物结合活性的生物学意义
  • 批准号:
    17570109
  • 财政年份:
    2005
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Regulation mechanism of tissue remodeling by gloycosylation change-Functional modulation of extracellular matrix molecule, vitronectin, by its glycosylation change
糖基化变化对组织重塑的调控机制——糖基化变化对细胞外基质分子玻连蛋白的功能调节
  • 批准号:
    12680607
  • 财政年份:
    2000
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Vitronectin expressed in porcine brain and characterization of lipid binding activities related to tissue vitronectins.
玻连蛋白在猪脑中的表达以及与组织玻连蛋白相关的脂质结合活性的表征。
  • 批准号:
    09680585
  • 财政年份:
    1997
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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肺病理学中细胞外基质和免疫细胞的相互作用:几丁质酶样蛋白的关键作用
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    24K21098
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    2024
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职业:工程水凝胶研究驱动细胞外基质重塑的宿主-寄生虫相互作用
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    2338708
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特发性肺纤维化中细胞外基质和细胞反应之间的机械化学相互作用(参考文献:4659)
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开发强大的天然细胞外基质,以改善胰岛功能,并减弱移植的免疫原性
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FTMA4 增强员工流动性以建设细胞外基质衰老研究和开发的能力
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