Regulation mechanism of nutrient assimilation and exocrine system which is achieved by the carbohydrate-recognition of pancreatic enzymes

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We found novel carbohydrate-binding activities of pancreatic enzymes; -amylase, trypsinogen/trypsin, and lipase, etc. The carbohydrate-binding sites of bovine trypsinogen and trypsin were identified by cocrystallization with Me -GalNAc, and X-ray crystallography. Expression, purification, and refolding methods of recombinant human pancreatic lipase were established for the first time using Escherichia coli. The interaction between -amylase and N-glycans in the BBM activated starch degradation to produce much more Glc on one hand, while suppressing a sharp increase in Glc absorption on the other. Therefore, the carbohydrate recognition of α-amylase was shown to playa key role in regulating Glc assimilation to maintain blood homeostasis in the intestine.