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Development of photo-driven motor proteins using phtochromic molecules

使用光致变色分子开发光驱动运动蛋白

基本信息

批准号：
14580677
负责人：
MARUTA Shinsaku
金额：
$ 2.24万
依托单位：
Soka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

项目摘要

ATP driven-motor proteins transduce chemical energy generated from ATP hydrolysis into the physical motion energy with the mechanical system like cam-shaft. It is expected that introduction of artificial energy input device into the energy transducing site of motor protein, enable the driving or regulating of motor proteins without ATP. In the present study, photochromic molecules which change their conformation reversibly with high response due to the isomerization by ultraviolet (UV) and visible light (VIS) irradiation, have been utilized as energy input device. Especially azobenzene and spiropyran derivatives, which change their characteristics dramatically on isomerization by UV-VIS light irradiation, might work effectively.In this study, several kinds of azobenzene and spiropyran derivatives, which can bind to cysteine side chain covalently, have been synthesized. And several motor protein mutants, which have one reactive cysteine at the specific site, have been also prepared. It has been demonstrated that for skeletal muscle myosin motor domain, the localized conformational changes resulting from isomerization on azobenzene derivative cross-linking of reactive cysteine residues, SH1 and SH2 induced swing in the lever arm portion. It was shown that the incorporated photochromic group acts as a mechanical energy input in a motor protein. The new technique using photochromic molecules may be applicable to other energy transducing and regulatory proteins.

ATP驱动马达蛋白通过凸轮轴等机械系统将ATP水解产生的化学能转化为物理运动能。在马达蛋白的能量传递位点引入人工能量输入装置，有望实现在无ATP的条件下对马达蛋白的驱动或调控。在本研究中，光致变色分子的构象可逆地改变与高响应，由于异构化的紫外线（UV）和可见光（维斯）照射，已被用作能量输入装置。特别是偶氮苯和螺吡喃衍生物，它们在紫外-可见光照射下的异构化特性会发生显著变化，因此可能是有效的。并制备了几种在特定位点有一个半胱氨酸活性的马达蛋白突变体。已经证明，对于骨骼肌肌球蛋白运动结构域，由偶氮苯衍生物交联反应性半胱氨酸残基、SH 1和SH 2的异构化引起的局部构象变化诱导杠杆臂部分的摆动。结果表明，掺入的光致变色基团在马达蛋白中充当机械能输入。利用光致变色分子的新技术可能适用于其他能量转换和调节蛋白质。