Structures, Permeabilties, and Cell Attachment and Growth of Tough and High Water Content PVA/(Water Soluble Polymer) Blend Hydrogels.

将聚乙烯醇（PVA）与另一种水溶性聚合物阴离子聚谷氨酸钠（PSLG）和聚乙烯磺酸钠盐（NaPSS）、阳离子壳聚糖、中性聚乙二醇（PEG）和聚丙烯酰胺（PAAm）的浓水溶液在高压釜中共混，制备了坚韧的橡胶样物理交联水凝胶。每一种冷冻混合水凝胶中都含有三种类型的水，即自由水的冰、未冻水和无序冰晶体。在未冻结的水凝胶中，冰的无序结晶作为水的中间态存在，分子间氢键减弱，分子迁移率低。PVA/NaPSS和PVA/壳聚糖水凝胶的氧渗透系数不依赖于水的含量，而是随着极窄渗透通道的半径和/或简单性的增加而增加，其中水分子的分子间氢键力减弱。而对于PVA/PEG和PVA/PAAm共混水凝胶，渗透系数取决于水凝胶的孔隙结构。壳聚糖含量低于10wt%的PVA/NaPSS、PVA/PEG、PVA/PAAm水凝胶和PVA/壳聚糖水凝胶几乎没有细胞附着。当壳聚糖含量超过20wt%时，细胞的附着和生长随壳聚糖含量的增加而增加，当壳聚糖含量达到40wt%时，水凝胶的含量超过胶原蛋白，被广泛用作哺乳动物细胞培养的底物。

Tough and rubber-like physically cross-linked hydrogels were prepared by blending of concentrated aqueous solutions of poly (vinyl alcohol) (PVA) and another water soluble polymer i. e. anionic poly (sodium L-glutamate) (PSLG) and poly (stirene sulfonic acid) sodium salt (NaPSS), cationic chitosan, and neutral poly (ethylene glycol) (PEG) and poly (acryl amide) (PAAm), in an autoclave.There are three types of water in every frozen blend hydrogel, i. e. ice of free water, unfrozen water, and disordered crystal of ice. In the unfrozen hydrogels, the disordered crystal of ice exists as an intermediate state of water which is weakened intermolecular hydrogen bonds and has low molecular mobility.The oxygen permeability coefficients of both the PVA/NaPSS and PVA/Chitosan hydrogels did not depend on the water content, and increase with increasing radius and/or simplicity of very much narrow permeable channels in which the water molecules has the weakened intermolecular hydrogen bonding force. While for the PVA/PEG and PVA/PAAm blend hydrogels the permeability coefficients depended on the porous structure in the hydrogels.Every PVA/NaPSS,PVA/PEG,and PVA/PAAm hydrogels and PVA/Chitosan hydrogels containing below 10wt% chitosan content were observed hardly any attached cells. While above 20wt% chitosan content the cell attachment and growth increases with increasing chitosan content and the hydrogel with 40wt% chitosan content exceed collagen which is widely used as a mammalian cell culture substrate.