Development of physiological status metrology of plant using chemoluminescence caused by active oxygen species

在这项研究中，我们尝试利用生物体发出的超弱光(称为生物光子)来实时检测各种刺激引起的生理状态的变化。生物光子是一种化学发光，源于线粒体呼吸过程中电子传递系统产生的电子对活性氧的激发。另一方面，这取决于细菌感染的应激强度或较强的环境应激。然后，将干旱胁迫、渗透压和食草性胁迫作为一种环境胁迫进行研究。我们发现，尽管干旱胁迫和盐胁迫在渗透胁迫点上是相同的，但生物光子的响应是不同的。此外，当水分再次供应给干燥的根时，观察到强烈的生物光子发射。因此，人们已经了解到能够根据逆境识别不同植物的生理状态。此外，随着应力的种类和损伤程度的不同，谱带强度随时间的变化也有较大差异。渗透胁迫下的发射强度越强，胁迫持续时间越长，植株生长越差。尤其是当细胞分裂区的发射强度较强时，无论个体差异如何，都会出现显著的生长受阻。此外，还研究了作为植物天敌的蜘蛛在草食胁迫下的生物光子发射。观察粉尘螨激发子诱导的发光，并实时检测其防御反应。

In this research, we have tried to use ultraweak light emitted from living organisms (called biophoton) to detect the change in the physiological status by various stimulation in real time. The biophoton is chemiluminescence originated from the active oxygen excited by the electron from the electron transport system along with the respiration in mitochondria. On the other hand, it depends on the strength of the stress of bacterial infection or the strong environmental stress. Then, the research was advanced paying attention to the drought stress, osmotic pressure, and the herbivorous stress as an environmental stress. We found that the response of a biophoton is different though the drought stress is the same as the salt stress in the point of osmotic stress. Moreover, when water is supplied to a dry root again, the strong biophoton emission is observed. Consequently, it has been understood to be able to identify the physiological status of a plant different according to the stress. In addition, the time-changing of the spectral band intensity has the large difference depending on the kind of the stress and the level of injury. The stronger the emission intensity under the osmotic stress was, the worse the growth of the plant after the stress duration was. Especially, the remarkable obstruction of growth occurred, when the emission intensity of the cell division area was strong regardless of the individual difference. Moreover, the biophoton emission was examined under the herbivorous stress caused by the spider mites that was insect enemies of plants. The luminescence induced by the elicitor of mites was observed, and the defense response was detected in real time.