Development of physiological status metrology of plant using chemoluminescence caused by active oxygen species

在本研究中，我们尝试使用生物体发出的超微弱光（称为生物光子）来检测真实的时间内各种刺激引起的生理状态变化。生物光子是线粒体呼吸过程中电子传递系统沿着传递的电子激发活性氧产生的化学发光。另一方面，它取决于细菌感染或环境胁迫的强度。然后，将干旱胁迫、渗透压和草食胁迫作为环境胁迫进行了研究。我们发现，从渗透胁迫的角度来看，干旱胁迫和盐胁迫对生物光子的响应是不同的。此外，当再次向干燥的根供水时，观察到强的生物光子发射。因此，已经理解能够根据胁迫来识别不同的植物的生理状态。此外，谱带强度随时间的变化因应力的种类和损伤程度的不同而有较大的差异。渗透胁迫下的排放强度越强，胁迫后植株的生长越差。特别是，当细胞分裂区的发射强度强而不考虑个体差异时，发生显著的生长障碍。此外，还研究了植物天敌叶螨引起的食草胁迫下的生物光子发射。观察螨类激发子诱导的发光，并真实的实时检测其防御反应。

In this research, we have tried to use ultraweak light emitted from living organisms (called biophoton) to detect the change in the physiological status by various stimulation in real time. The biophoton is chemiluminescence originated from the active oxygen excited by the electron from the electron transport system along with the respiration in mitochondria. On the other hand, it depends on the strength of the stress of bacterial infection or the strong environmental stress. Then, the research was advanced paying attention to the drought stress, osmotic pressure, and the herbivorous stress as an environmental stress. We found that the response of a biophoton is different though the drought stress is the same as the salt stress in the point of osmotic stress. Moreover, when water is supplied to a dry root again, the strong biophoton emission is observed. Consequently, it has been understood to be able to identify the physiological status of a plant different according to the stress. In addition, the time-changing of the spectral band intensity has the large difference depending on the kind of the stress and the level of injury. The stronger the emission intensity under the osmotic stress was, the worse the growth of the plant after the stress duration was. Especially, the remarkable obstruction of growth occurred, when the emission intensity of the cell division area was strong regardless of the individual difference. Moreover, the biophoton emission was examined under the herbivorous stress caused by the spider mites that was insect enemies of plants. The luminescence induced by the elicitor of mites was observed, and the defense response was detected in real time.