Development of physiological status metrology of plant using chemoluminescence caused by active oxygen species

在本研究中，我们尝试利用生物体发出的超弱光（称为生物光子）来实时检测各种刺激下生理状态的变化。生物光子是由线粒体内的电子传递系统中的电子激发活性氧产生的化学发光。另一方面，它取决于细菌感染的压力强度或较强的环境压力。然后，对干旱胁迫、渗透压胁迫和草食性胁迫作为环境胁迫进行了深入研究。我们发现，在渗透胁迫点上，尽管干旱胁迫与盐胁迫相同，但生物光子的响应是不同的。此外，当水分再次供给干根时，观察到强生物光子发射。因此，它已被理解为能够识别不同胁迫下植物的生理状态。不同的应力类型和损伤程度，谱带强度随时间变化有较大差异。渗透胁迫下的辐射强度越强，胁迫期后植株生长越差。特别是当细胞分裂区域的发射强度较强时，无论个体差异如何，都会发生明显的生长阻碍。此外，还研究了植物的天敌蜘蛛螨在草食胁迫下的生物光子发射。观察了螨虫激发剂诱导的发光，实时检测了防御反应。

In this research, we have tried to use ultraweak light emitted from living organisms (called biophoton) to detect the change in the physiological status by various stimulation in real time. The biophoton is chemiluminescence originated from the active oxygen excited by the electron from the electron transport system along with the respiration in mitochondria. On the other hand, it depends on the strength of the stress of bacterial infection or the strong environmental stress. Then, the research was advanced paying attention to the drought stress, osmotic pressure, and the herbivorous stress as an environmental stress. We found that the response of a biophoton is different though the drought stress is the same as the salt stress in the point of osmotic stress. Moreover, when water is supplied to a dry root again, the strong biophoton emission is observed. Consequently, it has been understood to be able to identify the physiological status of a plant different according to the stress. In addition, the time-changing of the spectral band intensity has the large difference depending on the kind of the stress and the level of injury. The stronger the emission intensity under the osmotic stress was, the worse the growth of the plant after the stress duration was. Especially, the remarkable obstruction of growth occurred, when the emission intensity of the cell division area was strong regardless of the individual difference. Moreover, the biophoton emission was examined under the herbivorous stress caused by the spider mites that was insect enemies of plants. The luminescence induced by the elicitor of mites was observed, and the defense response was detected in real time.