Development of the anti-oxidization activity measuring method using a few active oxygen species induced by microwave exposure and ultra weak bioluminescence

我们试图利用微波辐射产生的几个活性氧物种来测量生物的抗氧化活性，这些活性氧物种可以诱导出超微弱的生物发光(称为生物光子)。生物光子是源于活性氧物种的弱化学发光。生物体中的活性氧物种毒性很大，被生物体的抗氧化系统(某些酶和抗氧化剂)灭活。因此，如果我们能够在生物体中产生活性氧物种，我们就可以通过生物光子测量来测量其抗氧化活性。有研究人员报道，微波辐射诱导了一些活性氧物种。因此，我们制作了用于生物光子测量的微波辐射器，证实了微波辐射引起的生物光子发射的增加。此外，我们还测量了电加热器产生的热冲击下的生物光子发射，以研究微波辐射的主要影响。结果表明，温度升高越快，生物光子发射越强。因此，我们得出结论，微波辐射的主要作用是局部更快的加热。

We tried to measure the anti-oxidization activity of living organism using a few active oxygen species induced by microwave exposure that induce ultra weak bioluminescence (called biophoton). The biophoton is weak chemiluminescence originated from the active oxygen species. The active oxygen species are highly toxic, and inactivated by anti-oxidization system (some enzymes and antioxidants) of living organism. So if we can generate the active oxygen species in living organism, we can measure the anti-oxidization activity by the biophoton measurement.Some researchers report that microwave exposure induced some active oxygen species. Thus we made the microwave applicator for biophoton measurement, and confirmed the increment of biophoton emission induced by microwave exposure.Furthermore, we measured the biophoton emission under the heat shock occurred by an electric heater to investigate the heat shock that was the main effect of microwave exposure. As the results, faster temperature rise induced stronger biophoton emission. So, we concluded the main effect of microwave exposure was the localized quicker heating.