Software Development for the Effective Mouse Embryo Banking and Population Management System Utilizing Reproduction Technology

利用生殖技术开发有效的小鼠胚胎库和种群管理系统的软件

• 批准号: 15300144
• 负责人: NAKAGATA Naomi
• 金额: $ 9.28万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15300144/
• 关键词: Software; Reproduction technology; Genetically engineered mouse; Resource bank; Bar code management; Web application; IVF; Frozen preservation management; 胚・精子バンク; リソース; リレーショナルデータベース; ソフトウエア

The developed system is designed to consolidate information necessary for mouse banking system including :1.basic information, such as mouse strain name, background characteristics, and depositor information2.information before embryos are produced by perfusion in an in vitro and in vivo fertilization, such as superovulation, fertilization rate, and number of embryos3.thawing and recovery status information when the frozen embryos produced in step 2 are to be thawed and transferred, and transfer result information when they are transferred into the recipient4.isolator information including monitoring information, and5.storage information on the fertilized eggs producedThe system makes the most of the functions of relational database once data is input, it is reflected to the scheduling function in the system. Also, taking account of familiarity of the users, it adopted the layout similar to the paper form used before the system was introduced. Barcodes are used to manage the enormous a … More mount of frozen resources and minimize the human errors such as mix-up.In phase 2,we further pursued the user convenience by adopting the CARD ID to reinforce the relationship with the R-BASE, which has been open to the public, and accelerating the response to the outside. In addition, information on "embryo production" and "thawing and transfer," separately managed in phase 1,is now combined as a series of works and it became possible to manage package deposition requests. Furthermore, highly secure resource management has been realized by putting an ID sticker on top of frozen tubes, which used to be affixed with barcodes on their sides only.It is needless to say that we have contributed significantly to the effective utilization of existing assets by building a database system as a Web application. Such a complicated system used to be only possible with Windows computers, although most of the computers in this center are Macintosh. We succeeded in removing the barrier between different operating systems. Less

开发的系统旨在整合鼠标银行系统所需的信息，包括：1。基本信息，如小鼠品系名称、背景特征、寄主信息2.体外和体内受精灌注产生胚胎前的信息，如超排卵、受精率、胚胎数量3。步骤2中产生的冷冻胚胎进行解冻和移植时的解冻和恢复状态信息，以及移植到受者体内时的结果信息。隔离器信息包括监控信息，以及5。该系统充分利用了关系数据库的功能，一旦输入数据，就反映到系统的调度功能中。同时，考虑到用户的熟悉程度，采用了类似于系统引入之前使用的纸质表单的布局。条形码用于管理大量的冻结资源，并最大限度地减少人为错误，如混淆。在第二阶段，我们进一步追求用户的便利性，采用CARD ID来加强与已经对外开放的R-BASE的关系，并加快对外的响应。此外，在第一阶段单独管理的“胚胎生产”和“解冻和转移”信息现在合并为一系列工作，并且可以管理包装沉积请求。此外，通过在冷冻试管上粘贴ID标签，实现了高度安全的资源管理，而以前冷冻试管只在侧面贴上条形码。不用说，通过将数据库系统构建为Web应用程序，我们已经为有效利用现有资产做出了重大贡献。这样一个复杂的系统过去只能在Windows电脑上实现，尽管这个中心的大多数电脑都是Macintosh。我们成功地消除了不同操作系统之间的障碍。少

项目成果

熊大CARDにおける生殖工学技術研修について

关于熊本大学CARD生殖工程技术培训

• 发表时间: 2004
• 期刊: Experimental Animal Technology 39・2
• 作者: Nagai T;Yamada S;Tominaga T;Ichikawa M;Miyawaki A.;中潟 直己
• 通讯作者: 中潟 直己

中潟直己: "疾患モデル動物-病因解析での役割と限界 別冊・医学のあゆみ(編者:山村研一)"出版社:医歯薬出版. 130 (2004)

中形直树：“疾病模型动物 - 病因分析中的作用和局限性分册：医学史（编辑：山村健一）”出版：石药出版 130（2004）。