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Development of the high-resolution optical tomography capable for in vivo observation of biological tissue structure and the application to early-stage diagnosis of superficial cancer

开发可活体观察生物组织结构的高分辨率光学断层扫描技术及其在浅表性癌症早期诊断中的应用

基本信息

批准号：
15300162
负责人：
HARUNA Masamitsu
金额：
$ 5.76万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

Our research target is proposal and demonstration of a novel high-resolution optical coherence tomography(Hi-OCT) for early-stage diagnosis of stomach cancer. Our proposed technique is used for in-focus imaging method over the entire cross-sectional area of interest. This tomography has the spatial resolution of 3μm with the imaging depth of more than 3mm.(1)A mode-locked Ti : sapphire laser (femto-second laser) is used as the light source of the Hi-OCT. The coherence length of femto-second laser of 3.1μm was obtained by compensation of unbalance of chromatic dispersion of the low-coherence interferometer.(2)In order to obtain in-focus imaging over the entire cross-sectional area of interest, we used the focusing effect of the focusing lens of the sample. This method is called in-focus OCT, in which multiple OCT is made by shifting the focal plane of the objective inside of tissue, followed by piling up of the OCT images. The imaging depth of more than 3mm was successfully obtained.(3)High-speed OCT imaging is required for a real-time OCT with minimization of motion artifact. We proposed the OCT imaging system with the PZM delay line scanning, where two identical PZMs are driven in push-pull operation. The delay-line scanning speed was 100 scans/sec, and an OCT image consists of 400 scans, providing us the image data acquisition time of 4 sec.(4)In the gastrointestinal application, the Hi-OCT imaging can provide very clear imaging of muscularis mucosae, which is a bending film-like tissue of a few tens micron thickness, showing that our method is useful for the early-stage diagnosis of stomach cancer.

我们的研究目标是提出并演示一种用于胃癌早期诊断的新型高分辨率光学相干断层扫描（Hi-OCT）。我们提出的技术用于整个感兴趣横截面区域的聚焦成像方法。该断层扫描的空间分辨率为3μm，成像深度超过3mm。(1)采用锁模钛宝石激光器(飞秒激光器)作为Hi-OCT的光源。通过对低相干干涉仪色散不平衡的补偿，得到了3.1μm的飞秒激光相干长度。(2)利用样品聚焦透镜的聚焦效应，为了在整个感兴趣的横截面上获得聚焦成像。这种方法称为焦点OCT，通过在组织内部移动物镜的焦平面来进行多次OCT，然后将OCT图像叠加起来。成功获得了超过3mm的成像深度。(3)实时OCT需要高速OCT成像并最小化运动伪影。我们提出了具有 PZM 延迟线扫描的 OCT 成像系统，其中两个相同的 PZM 以推挽操作驱动。延迟线扫描速度为100次扫描/秒，一张OCT图像由400次扫描组成，为我们提供了4秒的图像数据采集时间。(4)在胃肠道应用中，Hi-OCT成像可以提供非常清晰的粘膜肌层成像，粘膜肌层是一种厚度为几十微米的弯曲薄膜状组织，表明我们的方法对于胃癌的早期诊断是有用的。

项目成果

期刊论文数量（120）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

In vivo observation of micro-tissue structures by high-resolution optical coherence tomography with a femtosecond laser

通过飞秒激光高分辨率光学相干断层扫描对微组织结构进行活体观察

DOI：
发表时间：
2004
期刊：
Trans.Jpn.Soc.Med.Bio.Eng. Vol.42
影响因子：
0
作者：
J.Nagayama;M.Nagayama;R.Nakagawa;H.Hirakawa;T.Matsueda;T.Iida;J.Fukushige;Masato Ohmi
通讯作者：
Masato Ohmi

High-resolution optical coherence tomography of biological tissue using a fiber-pigtailed thermal-light interferometer

使用尾纤热光干涉仪对生物组织进行高分辨率光学相干断层扫描