Detection of calcium by the nanosecond time-gated spectroscopy of plume in laser ablation of biological tissue and its application to clinical diagnoses

生物组织激光消融中羽流纳秒时间选通光谱检测钙及其在临床诊断中的应用

• 批准号: 11480256
• 负责人: HARUNA Masamitsu
• 金额: $ 5.18万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11480256/
• 关键词: Laser ablation of biological tissue; Nanosecond time-gated spectroscopy; Monitor of daily Ca intake; Optical coherence tomography (OCT); high-resolution OCT; Micrometer-scale optical diagnoses and surgeries; カルシウム検出; 骨粗鬆症診断; 光バイオプシ

We proposed nanosecond time-gated spectroscopy of plume in laser ablation of biological tissue, and suggested that this specific spectroscopy is useful for screening of osteoporosis and daily monitoring of Ca intake. On the basis of the research results, we have proposed micrometer-scale optical diagnoses and surgeries including effective diagnosis and removal of calcified malignant tumors.(1) We proposed the nanosecond time-gated spectroscopy of plume in tissue ablation. A prototype of spectroscopy system was developed to perform highly sensitive detection of calcium.(2) The Ca peak intensity was measured for several tens of hair samples of female over a wide range of age from infant to 90 years old. The Ca peak intensity became maximum around 15 years old, and then it decreased gradually. On the other hand, we did not recognize such a drastic change of the Ca peak intensity with age for male hairs.(3) It was found that there was a good relation of the Ca peak intensity and the measur … More ed value of bone mineral density with the dual-energy X-ray absorptiometry (DEXA), showing that our specific spectroscopy is applicable for screening of osteoporosis.(4) Ca detection was made for nail of rats with normal diet and Ca-deficient diet. Remarkable reduction of the Ca peak intensity was found for the Ca-deficient rats after ten-weeks old. On the contrary, there was no remarkable change of the Ca peak intensity of nail for ovariectomized rats, compared to that of control rats. This result suggested that our method is useful for monitor of daily Ca intake.(5) A new type of optical diagnosis and surgery with micrometer scale was proposed by combination of tissue ablation and optical coherence tomography (OCT), including measurement of Ca distribution along the skin depth and remove of calcified malignant tumor with the spatial resolution of the order of μm.(6) In the experiment, we measured Ca distribution along the radius for rat femur.(7) In order to improve the spatial resolution, high-resolution OCT of 1.3μm was successfully demonstrated using a ultra-low coherence interferometer where a halogen lamp was used as the light source. Less

我们提出了激光消融生物组织中羽流的纳秒时间门控光谱，并建议这种特定的光谱可用于骨质疏松症的筛查和钙摄入量的日常监测。在研究结果的基础上，我们提出了微米尺度的光学诊断和手术，包括钙化恶性肿瘤的有效诊断和切除。(1)提出了组织消融中羽流的纳秒时间门控光谱。开发了一种光谱系统的原型，用于高灵敏度的钙检测。(2)测量了从婴儿到90岁的几十个女性头发样品的Ca峰强度。Ca峰强度在15岁左右达到最大值，之后逐渐减弱。另一方面，我们没有发现男性头发的Ca峰强度随年龄的变化如此剧烈。(3)双能x线吸收仪（DEXA）的Ca峰强度与骨密度测量值有良好的关系，表明我们的特异光谱可用于骨质疏松症的筛查。(4)对正常饮食和缺钙饮食大鼠指甲进行钙检测。缺钙大鼠在10周龄后钙峰强度显著降低。相反，去卵巢大鼠指甲Ca峰强度与对照组相比无显著变化。结果表明，该方法可用于动物日钙摄入量的监测。(5)将组织消融与光学相干断层扫描（OCT）相结合，提出了一种微米尺度的新型光学诊断和手术方法，包括测量Ca沿皮肤深度的分布，并以μm量级的空间分辨率切除钙化恶性肿瘤。(6)在实验中，我们测量了大鼠股骨沿桡骨的Ca分布。(7)为了提高空间分辨率，采用卤素灯作为光源的超低相干干涉仪成功实现了1.3μm的高分辨率OCT。少

项目成果

近江 雅人: "低コヒーレンス光干渉による生体組織の屈折率と厚さ測定"医用電子と生体工学. Vol.37,No.1. 78-84 (1999)

Masato Omi：“通过低相干光学干涉测量生物组织的折射率和厚度”《医疗电子和生物工程》第 37 卷，第 78-84 期（1999 年）。

Hideki Maruyama: "A practical measurement system for determination of refractive index and thickness using the low coherence interferometry"1999 Int'l Conf.Opt.Eng.for Sensing and Nanotech.(ICOSN'99). Vol.3740. 26-29 (1999)

Hideki Maruyama：“使用低相干干涉测量法确定折射率和厚度的实用测量系统”1999 Intl Conf.Opt.Eng.for Sensing and Nanotech.(ICOSN99)。

Masamitsu Haruna: "Optical reflection imaging for display of 2-D refractive index distribution along the geometrical thickness"OSA Biomed.Topical Meetings,Tech.Digest. MB5. 194-196 (2000)

Masamitsu Haruna：“用于显示沿几何厚度的二维折射率分布的光学反射成像”OSA Biomed.Topical Meetings，Tech.Digest。

春名 正光: "生体のマイクロ光診断と実サイズ光トモグラフィ"O plus E. Vol.23, No.1. 86-89 (2001)

Masamitsu Haruna：“生物体的显微光学诊断和真实尺寸光学断层扫描”O plus E. Vol.23，No.1（2001）。

Masamitsu Haruna: "Optical tomography in the medical field, Optical tomography by the low coherence interferometry"O plus E. Vol.21, No.7. 800-808 (1999)

Masamitsu Haruna：“医学领域的光学断层扫描，低相干干涉测量法的光学断层扫描”O plus E. Vol.21，No.7。