Mutual regulatory mechanism between Ca2+ permeable receptors and channels and Ca2+ binding proteins

Ca2+通透性受体和通道与Ca2+结合蛋白之间的相互调节机制

• 批准号: 15500223
• 负责人: OKADO Haruo
• 金额: $ 2.3万
• 依托单位: Tokyo Metropolitan Organization for Medical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15500223/
• 关键词: Glutamate receptor; calbindinD28k; AMPA receptor; CREB; calcium binding protein; カルシウム透過型AMPA受容体; CalbindinD28k; NMDA受容体; カルシウム結合蛋白; paravalbumin; AMPA受容体; カルビンジン; CREB

We reported that the expression of Ca2+ binding proteins, calbindin-D28k (CB) and parvalbumin correlates with the expression of Ca2+ permeable AMPA receptors (AMPA-Rs) in rat forebrain. We analyzed cell surface expression of a Ca2+ impermeable subunit, GluR2 and a Ca2+ permeable subunit, GluR2Q using an adenoviral-mediated expression system in COS7 cells. Biochemical measurements of cell surface expressed subunits using biotinylation experiments revealed that cell surface expression of GluR2Q is significantly increased with CB coexpression. By application of AMPA-R blockers, cell surface expression of GluR2Q is increased in the absence of CB coexpression. To determine whether Ca2+ entry via Ca2+ permeable AMPA receptor causes the suppression, we used the Ca2+ chelater, BAPTA-AM. Application of BAPTA-AM increases cell surface expression of GluR2Q. These results suggest that CB enhances cell surface expression of Ca2+ permeable AMPA-Rs by buffering the increased intracellular Ca2+ that enters through Ca2+ permeable AMPA-Rs.

本研究报道了大鼠前脑Ca ~（2+）结合蛋白calbindin-D28 k（CB）和parvalbumin的表达与Ca ~（2+）渗透性AMPA受体（AMPA-Rs）的表达相关。我们使用腺病毒介导的表达系统在COS 7细胞中分析了Ca 2+不可渗透亚基GluR 2和Ca 2+可渗透亚基GluR 2 Q的细胞表面表达。使用生物素化实验的细胞表面表达的亚基的生化测量显示，细胞表面表达的GluR 2 Q与CB共表达显着增加。通过应用AMPA-R阻断剂，在CB共表达不存在的情况下，GluR 2 Q的细胞表面表达增加。为了确定Ca 2+通过Ca 2+渗透性AMPA受体进入是否引起抑制，我们使用了Ca 2+螯合剂BAPTA-AM。BAPTA-AM的应用增加了GluR 2 Q的细胞表面表达。这些结果表明，CB通过缓冲通过Ca 2+渗透性AMPA-Rs进入的增加的细胞内Ca 2+来增强Ca 2+渗透性AMPA-Rs的细胞表面表达。

项目成果

Distinct aggregation and cell death patterns among different types of primary neurons induced by mutant huntingtin protein

• DOI: 10.1111/j.1471-4159.2004.02372.x
• 发表时间: 2004-05
• 期刊: Journal of Neurochemistry
• 影响因子: 4.7
• 作者: K. Tagawa;M. Hoshino;T. Okuda;H. Ueda;H. Hayashi;S. Engemann;H. Okado;M. Ichikawa;E. Wanker;H. Okazawa

Newly-formed axonal branches of rat sciatic neurons sprouting in the spinal cord after peripheral axotomy.

周围轴突切除术后，大鼠坐骨神经元新形成的轴突分支在脊髓中萌芽。

• 发表时间: 2004
• 期刊: Scand J Plast Reconstr Surg Hand Surg 38(3)
• 作者: Zenzai K;Shibata M;Okado H;Endo N;Hirano S.
• 通讯作者: Hirano S.

Calbindin-D28k coexpression enhances cell surface expression of Ca2+ permeable AMPA receptors

Calbindin-D28k 共表达增强 Ca2 渗透性 AMPA 受体的细胞表面表达

• 发表时间: 2004
• 期刊: Japanese Journal of Physiolog 54
• 作者: Takahashi A;Kondo M;Miwa A;Okado H
• 通讯作者: Okado H

Saito et al.: "Transfer of NMDAR2 cDNAs increases endogenous NMDAR1 protein and induces expression of functional NMDA receptors in PC12 cells."Brain Res Mol Brain Res.. 110. 159-168 (2003)

Saito 等人：“NMDAR2 cDNA 的转移会增加内源性 NMDAR1 蛋白并诱导 PC12 细胞中功能性 NMDA 受体的表达。”Brain Res Mol Brain Res.. 110. 159-168 (2003)

Sequential exocytosis of insulin granules is associated with redistribution of SNAP25.

• DOI: 10.1083/jcb.200312033
• 发表时间: 2004-04-26
• 期刊: The Journal of cell biology
• 作者: Takahashi N;Hatakeyama H;Okado H;Miwa A;Kishimoto T;Kojima T;Abe T;Kasai H
• 通讯作者: Kasai H