calcium-binding protein and glutamatergic neurons

钙结合蛋白和谷氨酸能神经元

• 批准号: 18500257
• 负责人: OKADO Haruo
• 金额: $ 2.63万
• 依托单位: Tokyo Metropolitan Organization for Medical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18500257/
• 关键词: RP58; cerebral cortex; calretini; 転写抑制因子; Calretinin; アポトーシス; カルビンジン

Cerebral Cortex is a brain structure that is developed in the higher orgasm, and function in the memory formation and cognition Recently, we identified a novel transcription factor POZ-zinc finger protein, RP58. We found that this transcript is expressed predominantly in the cerebral cortex in the embryonic stage. In particular, it is expressed faintly in the VZ and strongly in the subventricular zone and cortical plate region. To understand the function of RP58, we generated the RP58-deficient mice. The RP58-null mouse shows the hypoplasia of cerebral cortex and cerebellum. In the cerebral cortex, the formation of subplate layer is markedly retarded. The formation of inside-out pattern also retarded. The double-labeling with IdU and BrdU suggested that the generation of the late-born neurons is delayed. Furthermore, the late-born neurons show abnormal enhancement of calrtinin-immunoreactivity Reporter-assay experiment suggested the RP58 directly supressed the expression of calretinin. These results suggested that RP58 is essential for normal generation of the late-born neurons via the regulation of neurogenin expression.

大脑皮层是一种大脑结构，在较高的性高潮中发育，并且在记忆形成和认知中起作用，我们确定了一种新型的转录因子poz-zinc手指蛋白RP58。我们发现该转录本主要在胚胎阶段的大脑皮层中表达。特别是，它在VZ中微弱地表达，在室内和皮质板区域中强烈表达。为了了解RP58的功能，我们生成了RP58缺陷小鼠。 RP58无效的小鼠显示了大脑皮层和小脑的发育不全。在大脑皮层中，子板层的形成显着延迟。内而外模式的形成也迟缓。与IDU和BRDU的双重标记表明，后期神经元的产生延迟了。此外，后期神经元显示出异常的calrtinin-rumunoreactitivitivitivitivitivitivitivitivitivitivitivitivitivitivitive retorter-sase-aysay实验表明，RP58直接抑制了钙蛋白素的表达。这些结果表明，RP58对于通过调节神经蛋白蛋白表达的正常生成后期神经元至关重要。

项目成果

Co-localization of a nivel transcriptional repressor sismiRP58 with RP58

nivel 转录抑制因子 sismiRP58 与 RP58 的共定位

• 发表时间: 2008
• 期刊: Biochem.Biophys.Res.Comm. 368
• 作者: Takahashi;et. al.
• 通讯作者: et. al.

カルビンディン遺伝子導入はMPP+による培養黒質ドーパミンニューロンの脱落を阻止する,

钙结合蛋白基因转移可防止 MPP+ 诱导的培养黑质多巴胺神经元脱落。

• 发表时间: 2007
• 作者: 西克 典;高田 昌彦;岡戸 晴生;三輪 昭子
• 通讯作者: 三輪 昭子

Retrograde infection of precerebellar nuclei neurons by injection of a recombinant adenovius into the cerebellar cortex of nomal and reeler mice.,(2007-04),51-62

将重组腺病毒注射到正常小鼠和 reeler 小鼠的小脑皮质中逆行感染小脑前核神经元。,(2007-04),51-62

• 发表时间: 2007
• 期刊: Arch Histol Cytol, 70(1)
• 作者: Tanaka;K.;Okado;H.;Terashima;T
• 通讯作者: T

Spatial and temporal expression of RP58, a novel zinc finger transcriptional repressor, in mouse brain

• DOI: 10.1002/cne.21350
• 发表时间: 2007-06-20
• 期刊: JOURNAL OF COMPARATIVE NEUROLOGY
• 影响因子: 2.5
• 作者: Ohtaka-Maruyama, Chiaki;Miwa, Akiko;Okado, Haruo
• 通讯作者: Okado, Haruo

Glia Nax channels control lactate signaling to neurons for brain[Na+]sensing.,

神经胶质 Nax 通道控制乳酸信号传导至神经元以进行大脑 [Na] 传感。

• 发表时间: 2007
• 期刊: Neuron 54(1)
• 作者: Shimiz;H.;Watanabe;E.;Hiryama;T. Y.;Nagakura;A.;Fujisawa;A.;Okado;H.;Yanagawa;Y.;Noda;M.
• 通讯作者: M.