The analysis of turbulent flow shear stress-responsive genes in vascular endothelial cells

血管内皮细胞湍流剪切应力响应基因分析

• 批准号: 15500323
• 负责人: OHURA Norihiko
• 金额: $ 2.3万
• 依托单位: Saitama medical school
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15500323/
• 关键词: DNA microarray; Shear stress; Turbulent flow; Laminar flow; Shear-stress-responsive genes; Endothelial cell; DNAマイクロアレイ解析; 乱流性シェアストレス; 流れ負荷装置

DNA microarray gene expression analysis was conducted in human umbilical vein endothelial cells (HUVECs) and coronary artery endothelial cells (HCAECs) exposed to laminar or turbulent shear stress. Approximately 3% of the total 5600 gene in HUVECs and HCAECs increased their expression more than two-fold or decreased it to less than half the static control in response to an arterial level of laminar shear stress (15 dynes/cm(2) for 24 hours). The proportions of shear-stress-responsive genes decreased to around 2% under the venous level of laminar shear stress (1.5 dynes/cm(2)) in both cell lines. Turbulent shear stress of 1.5 dynes/cm(2) altered the expression of 1.1% of all genes in the HCAECs. Laminar shear stress, but not turbulent shear stress, decreased the expression of a number of genes involved in DNA synthesis and the cell cycle in both HUVECs and HCAECs. Clustering analysis showed a variety of temporal profiles of gene expression in HUVECs exposed to laminar shear stress of 15 dynes/cm(2) for 3, 6, 12, 24, and 48 hours. Turbulent shear stress affected expression of many genes that play a role in vascular remodeling, including genes encoding plasminogen activators and their inhibitor, endothelin-1, transforming growth factor-beta, collagen type IV, and ephrin A1.

应用DNA微阵列技术分析了层流和湍流切应力作用下人脐静脉内皮细胞（HUVECs）和冠状动脉内皮细胞（HCAECs）的基因表达。HUVEC和HCAEC中约3%的5600个基因在动脉水平的层流剪切应力（15达因/cm（2），持续24小时）下表达增加2倍以上或减少至静态对照的一半以下。在静脉水平的层流剪切应力（1.5达因/cm（2））下，两种细胞系中剪切应力反应基因的比例下降到2%左右。1.5 dynes/cm（2）的湍流剪切应力改变了HCAEC中1.1%的所有基因的表达。层流剪切应力，但不是湍流剪切应力，减少了一些基因的表达，参与DNA合成和细胞周期在HUVEC和HCAEC。聚类分析显示，HUVECs暴露于15 dynes/cm（2）的层流剪切应力3，6，12，24和48小时的基因表达的各种时间概况。湍流切应力影响了许多在血管重塑中发挥作用的基因的表达，包括编码纤溶酶原激活物及其抑制剂、内皮素-1、转化生长因子-β、IV型胶原蛋白和肝配蛋白A1的基因。

项目成果

Global analysis of shear stress-responsive genes in vascular endothelial cells

血管内皮细胞剪切应力反应基因的整体分析

• 发表时间: 2003
• 期刊: J. Atheroscler Thromb. 10(5)
• 作者: Ohura N;Ando J
• 通讯作者: Ando J

粘着型ハイドロセルラードレッシングの褥瘡治療

水细胞粘性敷料治疗压疮

• 发表时间: 2004
• 期刊: 日本褥瘡学会誌 6(1)
• 作者: 大浦紀彦;市岡滋;中塚貴志
• 通讯作者: 中塚貴志

Evaluation of hydrocellular adhesive dressing for Pressure ulcers

水细胞粘合敷料治疗压疮的评价

• 发表时间: 2004
• 期刊: Jpn J PU. 6(1)
• 作者: Ohura N;Ichioka S;Shibata M;Nakatsuka T
• 通讯作者: Nakatsuka T

Norihiko Ohura et al.: "Global Analysis of Shear Stress-Responsive Genes in Vascular Endothelial Cells"J.Atherosclerosis Thrombosis. 10・5. 304-313 (2003)

Norihiko Ohura 等：“血管内皮细胞中剪切应力响应基因的整体分析”J. 动脉粥样硬化血栓形成 10・5 (2003)。

Global analysis of shear stress-responsive genes in vascular endothelial cells.

血管内皮细胞中剪切应力响应基因的整体分析。

• 发表时间: 2003
• 期刊: J.Atheroscler Thromb. 10(5)
• 作者: Ohura N;Ando J.
• 通讯作者: Ando J.