Process for formation of chromatin functional domains using Drosophila

使用果蝇形成染色质功能域的过程

• 批准号: 15510162
• 负责人: HIROSE Fumiko
• 金额: $ 2.05万
• 依托单位: University of Hyogo (2004)Himeji Institute of Technology (2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15510162/
• 关键词: Drosophila melanogaster; chromatin domain; transcription factor; chromatin remodeling factor

Transgenic fly lines carrying UAS-BEAF and UAS-BEAF RNAi have been established in addition to the lines carrying UAS-DREF and UAS-Mi-2. Using these lines, over-expression or knock-down of BEAF protein is realized in tissues-specific manner. Over-expression of BEAF protein in cells of the eye-imaginal disc resulted in rough-eye phenotype. On the other hand, when BEAF transcript was reduced by BEAF-specific double-stranded RNA in cells of the eye imaginal disc or wing disc, morphology of adult eye or wing seems to be normal.. Reducing level of BEAF protein in early embryo caused retardation of development and all of animals were dead at first instar larval stage. These result suggest that BEAF might have important roles in early stages of development but in the later stages after 2^<nd> or 3^<rd> -instar larva.Recruitment of DREF, BEAF, and Mi-2 at the genes for heat shock protein 70 (hsp70) were analyzed before and after heat shock.It is well known that treatment of animals at 37 C establishes special chromatin structure around the hsp70 gene and strong and induces transient transcription of the hsp70 gene, but little is known about factors involved in formation of the special chromatin domain and activation of transcription of the hsp70 gene locus. Both DREF and Mi-2 were rapidly recruited at the hsp70 locus after heat shock.. Now, we are preparing system for examining what is happened at the hsp70 locus and what roles DREF and Mi-2 have in that phenomena.

除了携带UAS-DREF和UAS-Mi-2的系外，还建立了携带UAS-BEAF和UAS-BEAF RNAi的转基因蝇系。利用这些细胞系，以组织特异性的方式实现了BEAF蛋白的过表达或敲低。BEAF蛋白在眼象盘细胞中的过度表达导致眼粗糙表型。另一方面，当BEAF转录物被BEAF特异性双链RNA在眼视盘或翼盘细胞中还原后，成人眼或翼的形态似乎是正常的。早胚BEAF蛋白水平降低导致发育迟缓，所有动物均在1龄幼虫期死亡。这些结果表明，BEAF可能在早期发育阶段发挥重要作用，但在2^<和>或3^<和>龄期幼虫的后期发育阶段发挥重要作用。分析热休克前后DREF、BEAF和Mi-2在热休克蛋白70 （hsp70）基因上的募集情况。众所周知，37℃的动物处理在hsp70基因周围建立了特殊的染色质结构，并强烈诱导了hsp70基因的瞬时转录，但对特殊染色质结构域的形成和hsp70基因位点转录激活的相关因素知之甚少。热休克后，DREF和Mi-2均在hsp70位点快速募集。现在，我们正在准备系统来检查hsp70位点发生了什么以及DREF和Mi-2在这种现象中起什么作用。

项目成果

DREF is required for EGFR signalling During Drosophila wing vwin development.

在果蝇 wing vwin 发育过程中，EGFR 信号转导需要 DREF。

• 发表时间: 2004
• 期刊: Genes Cells 10
• 作者: Yoshida H.et al.;Yamashita D.et al.;Yoshida H. et al.
• 通讯作者: Yoshida H. et al.

Genetic link between p53 and genes required for formation of the zonula adherens junction

• DOI: 10.1111/j.1349-7006.2004.tb03228.x
• 发表时间: 2004-05
• 期刊: Cancer Science
• 影响因子: 5.7
• 作者: M. Yamaguchi;F. Hirose;Y. Inoue;K. Ohno;H. Yoshida;Y. Hayashi;P. Deák;A. Matsukage

Choi TY.et al.: "Redox regulation of DNA binding activity of DREF (DNA replication-related element binding factor) in Drosophila"Biochem.J.. 378. 833-838 (2004)

Choi TY.等：“果蝇中DREF（DNA复制相关元件结合因子）DNA结合活性的氧化还原调节”Biochem.J. 378. 833-838（2004）

DREF is required for EGFR signalling during Drosophila wing vein development

• DOI: 10.1111/j.1365-2443.2004.00775.x
• 发表时间: 2004-10
• 期刊: Genes to Cells
• 影响因子: 2.1
• 作者: H. Yoshida;E. Kwon;F. Hirose;Kyoko Otsuki;Mikihiro Yamada;M. Yamaguchi

Yamaguchi M.et al.: "Genetic link between p53 and genes required for formation of the zonula adherens junction"Cancer Sci.. (in press).

Yamaguchi M.et al.：“p53 与形成粘附小带连接所需基因之间的遗传联系”癌症科学（正在出版）。