Development of a simple method for monitoring air-borne fungi in houses

开发一种监测房屋空气真菌的简单方法

基本信息

批准号：
15560517
负责人：
IWATA Toshie
金额：
$ 1.34万
依托单位：
Tokai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

Air-borne fungi cause health problems e.g. allergic asthma. Since there are various methods to measure the concentration of air-borne fungi, it is difficult to compare the results measured by different measurement methods. In this study, a simple monitoring method which is cheap and easy to handle has been developedUsing existing air samplers the reproducibility, the effects of sampling volume and airflow on the sampling efficiency were examined and the relationships between the samplers were identified. The accuracy which was required for measurement of air-borne fungi was also identified. The basic concept of the method was determined in which Air-borne fungi spores adhering on the vertical sampling faces are collected on agar plate. It was found that the number of CFU with PET was the highest and the coefficient of variance of the number of CFU with PET was the lowest. The difference between the conductors was not significant.The sampling efficiency of the simple method was defined. From the results of the measurements, the suitable exposure time was expected from one week to two weeks. The effects of air velocity against the sampler could be disregarded in case the air velocity was lower than 0.5m/s. the effects of electrical charged amount on the sampler surface on sampling efficiency could be disregarded in case the relative humidity was higher than 35%.In order to reduce invalid samplings which are caused by inadequate sterilization of samplers before exposure and individual difference in pressure, the method was modified by using a sample case filled with ethanol vapor and weight for 25 g/cm^2 of pressure.This modification reduces percentage of invalid samples to 20%.

空中真菌引起健康问题，例如过敏性哮喘。由于有多种方法可以测量真菌的浓度，因此很难比较通过不同测量方法测量的结果。在这项研究中，已经开发了一种简单的监测方法，该方法已经开发出现有空气采样器的可重复性，采样量和气流对采样效率的影响，并确定了采样器之间的关系。还确定了测量空气真菌所需的准确性。该方法的基本概念是确定的，其中在琼脂板上收集了粘附在垂直采样面上的空气真菌孢子。发现具有PET的CFU的数量最高，并且PET的CFU数量的差异系数最低。导体之间的差异并不显着。定义了简单方法的采样效率。从测量结果中，预计合适的曝光时间是一周到两周。如果空气速度低于0.5m/s，则可以忽略空气速度对采样器的影响。如果相对湿度高于35％，则可以忽略电荷电荷量对采样效率的影响。为了减少无效采样，这是由于暴露之前的采样器不足而导致的无效采样，并且使用乙醇蒸发和重量的样本范围填充了25 g/cm的压力，该方法是通过压力来修改的。样本至20％。