The Development of Ddegradation System for Dioxins Using a New White Rot Fungus

1利用旋转圆盘生物反应器，利用担子菌（Coprinus cinereus）培养进行了生产酶-过氧化物酶的实验。利用得到的粗过氧化物酶，对壬基酚和双酚A的脱除工艺进行了优化。壬基酚可通过氧化酶反应降解，但降解机理与双酚A不同。双酚A可通过氧化聚合沉淀法去除本研究的主题是利用新筛选的白腐菌开发降解二恶英的生物修复技术。作者对二恶英降解微生物进行了筛选，并在1000株菌株中筛选出了一株具有高降解性的白腐菌L-25。通过对该微生物16rDNA-500序列的分析，我们发现它是一个与原毛菌属有关的新菌株。对该菌株进行了不同条件下的培养，获得了大量的锰过氧化物酶。此外，添加蛋白胨后，锰过氧化物酶的产率较高，可产锰过氧化物酶14 U/mL。此外，以二恶英为底物，对该菌株进行培养，发现100 ng/mL的1,3,7,8-四氯二恶英或1,2,7,8-四氯二苯并呋喃在一周内降解率超过60%。二恶英的降解速度比以前的报告要快。

1 With a rotating disk bioreactor, the experiment to produce the enzyme -peroxidase-was carried out using a basidiomycete (Coprinus cinereus) culture. Using the crude peroxidase obtained, the treatments to remove nonylphenol and bisphenol A were optimized. Nonylphenol was degraded by an oxidative enzyme reaction, but the mechanism of degradation was different from that of bisphenol A. Bisphenol A was removed by the method of an oxidative polymerization and precipitation.2 The subject of this research is to develop a bioremediation technique for the degradation of dioxins using new screened white rot fungi. The authors attempted to the screening of dioxin degradable microorganisms, and among 1000 strains, a new white rot fungus L-25 strain was screened due to its high degradability. Analyzing the 16rDNA-500 arrangement of this microorganism, we found that it was a new strain related to the genus Phanerochaete. The culture of this strain was carried out at various conditions, and a lot of manganese peroxidase was obtained. Furthermore, adding peptones, a high productivity was obtained, and 14 U/mL of manganese peroxidase was produced. In addition, using dioxins as a substrate and culturing this strain, it was found that more than 60 % of 1,3,7,8-tetrachlorodioxin or 1,2,7,8-tetrachlorodibenzofuran with 100 ng/mL was degraded for one week. This rate of degradation for dioxins is faster than the previous reports.