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Removal of Phenolic compounds with an endocrine-disrupting effect by Polymerization and Precipitation Method Using Microbial Peroxidase

利用微生物过氧化物酶聚合沉淀法去除具有内分泌干扰作用的酚类化合物

基本信息

批准号：
13650853
负责人：
SAKAKIBARA Mikio
金额：
$ 0.83万
依托单位：
Fukui University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

1. The peroxidase (a lignin degrading enzyme) production by Coprinus cinereus using a rotating disk contactor was examined for the purpose of the producing the crude enzyme, and the effect of an inducer on peroxidase production was also investigated. As an inducer, no results were obtained using syringaldazine, but when hydrogen peroxide or 5-amino-levulinic acid were used as the additive to the culture medium, the suitable effectiveness was obtained. For example, adding the inducer into the 3 ^<rd> day culture, the enzyme activity was increased 1.8-2 times (100U/ml). Further, the optimum dosage of hydrogen peroxide was 0.1-0.2 mM. In future, we must consider the effectiveness of the inducer in a repeated batch culture.2. The reaction conditions on phenolic compounds (including an endocrine-disruptor) removal by the polymerization and precipitation method using the microbial peroxidase were investigated. Consequently, the optimum stoichiometric relationship between peroxidase, phenolic compounds and hydrogen peroxide, and the effect of pH and temperature on removal efficiency were examined. The most effective ratio of hydrogen peroxide to phenol was nearly 1/1 (mol/mol) at an adequate enzyme dose, and 12-14 U of the enzyme was needed to remove 1 mg of pehnol. At an insufficient peroxidase dose, the optimum pH value was 9.0, and lowering the reaction temperature led to the improvement of removal efficiency. Moreover, adding non-ionic surfactant (Triton X-100), the required amount of peroxidase to remove phenol was reduced about 1/4. The reaction conditions on bisphenol A removal presented the similar behavior compared to the cases of phenol, but the required amount of peroxidase and reaction time were 4-5 times.

1. 以转盘接触器为原料，考察了灰鸡鸡生产过氧化物酶（木质素降解酶）的工艺条件，并考察了诱导剂对过氧化物酶生产的影响。以紫丁香嗪作为诱导剂，诱导效果不佳，以双氧水或5-氨基乙酰丙酸作为培养基的添加剂，诱导效果较好。例如，将诱导剂添加到3 ^< 1 / 3 >天的培养中，酶活性提高1.8-2倍（100U/ml）。此外，过氧化氢的最佳用量为0.1-0.2 mM。未来，我们必须考虑诱导剂在重复分批培养中的有效性。研究了微生物过氧化物酶聚合沉淀法去除含内分泌干扰物的酚类化合物的反应条件。研究了过氧化物酶、酚类化合物和过氧化氢的最佳化学计量关系，以及pH和温度对过氧化氢脱除效果的影响。在适当的酶量下，过氧化氢与苯酚的最有效比接近1/1 (mol/mol)，去除1 mg苯酚需要12-14 U的酶。过氧化物酶用量不足时，最佳pH值为9.0，降低反应温度可提高脱除效率。此外，添加非离子表面活性剂Triton X-100后，过氧化物酶去除苯酚所需的量减少了约1/4。双酚A脱除的反应条件与苯酚相似，但所需的过氧化物酶量和反应时间是苯酚的4-5倍。