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Analyses of responsible genes of morphological mutants in the Japanese morning glory.

日本牵牛花形态突变体的相关基因分析。

基本信息

批准号：
15570007
负责人：
NITASAKA Eiji
金额：
$ 2.37万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570007/
关键词：
Ipomoea nil FEATHERED morphogenesis transposable elements KAN1 Japanese morning glory petaloid

项目摘要

The semi-dominant feathered (fe) mutants in the Japanese morning glory have strongly crumpled or rolled leaves, and flowers with deeply split or tube-like petals folding back at their tips. Histological analysis showed that the number of stomata in abaxial side decreased in fe mutants. These mutant Phenotypes suggest that FE is involved in establishment of organ polarity. We isolated a rare fe revertant caused by excision of a transposable element inserted in FE gene. Tpn1 family which belong to En/Spn related transposable elements is known to induce most mutations in the Japanese morning glory. FE gene was isolated using Simplified Transposon Display(STD) method which allows visualization and isolation of Tpn transposon-flanking sequences from high copy-number lines. FE gene encodes a protein in the GARP family of putative transcription factors, and has high homology with KANADI1(KAN1) gene of Arabidopsis. We suppose that FE expresses on abaxial side of lateral organs, and specify the … More abaxial identity as KAN. Gentic and molecular analyses showed that the strong fe phenotype is caused by an additional mutation occurred in fe background.The prtaloid(pt) mutants have petaloid stamens and short sepaloid carpel. This phenotype seems to be caused by the mutation of a C-function floral homeotic gene. Mutations of duplicated(dp) cause a substitution of reproductive organs to perianth organs (petals and sepals). An En/Spm-related transposable element, Tpn-botan, was inserted in the second intron of a C-funtion MADS-box gene DP and the subsequent excision event led to a deletion of a substantial part of the original Tpn element and DP genome (dp-1 allele). We assumed that pt is a week DP allele and examined the genomic structure of the second intron of DP gene in pt mutants. A full length Tpn 109 was inserted in the same place of dp-1 allele in the pt mutant. It is known that pt phenotypes become weaker during shoot growth, but Tpn 109 insertion was still obeserved in weak phenotype flower. Therefore, pt phenotype is caused by reduction of DP gene transcripts by low splicing efficiency or split cis-regulatory elements. To examine the deletion mechanism mediated by Tpn transposon, I analyzed genomic structures of other two dp alleles (dp-2, dp-3). dp-2 and dp-3 alleles have two and three rearranged Tpn 109 elements, respectively, and genomic region between two Tpn 109 elements was deleted. Less

日本牵牛花的半显性羽状（fe）突变体具有强烈的褶皱或卷曲的叶子，花朵具有深裂或管状花瓣，花瓣在其尖端折叠。组织学分析表明fe突变体远轴侧气孔数量减少。这些突变表型表明FE参与器官极性的建立。我们分离出一种罕见的fe回复突变体，该突变体是由插入FE基因中的转座因子的切除引起的。Tpn1家族属于En/Spn相关的转座因子，已知其在日本牵牛花中诱导大多数突变。使用简化转座子展示（STD）方法分离FE基因，该方法允许从高拷贝数系中可视化和分离Tpn转座子侧翼序列。FE基因编码GARP家族中的一个蛋白质，与拟南芥的KANADI1（KAN1）基因具有高度同源性。我们假设FE表达在侧器官的远轴侧，并明确了FE的表达部位。 ...更多信息作为KAN的远轴身份。遗传和分子生物学分析表明，强fe表型是在fe背景下发生的一个额外突变所致，突变体为花瓣状雄蕊和短萼片状心皮。这种表型似乎是由C功能花同源异型基因突变引起的。复制（dp）突变导致生殖器官被花被器官（花瓣和萼片）取代。一个En/Spm相关的转座因子Tpn-botan被插入到C功能MADS盒基因DP的第二个内含子中，随后的切除事件导致原始Tpn因子和DP基因组的大部分缺失（dp-1等位基因）。我们假设pt是一个弱DP等位基因，并检测了pt突变体DP基因第二内含子的基因组结构。在pt突变体中，在dp-1等位基因的相同位置插入全长Tpn 109。在新梢生长过程中，pt表型逐渐减弱，但在弱表型花中仍能观察到Tpn 109插入。因此，pt表型是由低剪接效率或分裂顺式调控元件引起的DP基因转录物减少引起的。为了研究Tpn转座子介导的缺失机制，我分析了另外两个dp等位基因（dp-2，dp-3）的基因组结构。dp-2和dp-3等位基因分别含有2个和3个重排的Tpn 109元件，两个Tpn 109元件之间的基因组区域缺失。少

项目成果

期刊论文数量（28）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Japanese morning glory dusky mutants displaying reddish-brown or purplish-gray flowers are deficient in a novel glycosylation enzyme for anthocyanin biosynthesis, UDP-glucose : anthocyanidin 3-O-glucoside-2"-O-glucosyltransferase, due to 4-bp insertions i

显示红棕色或紫灰色花朵的日本牵牛花暗色突变体缺乏用于花青素生物合成的新型糖基化酶，UDP-葡萄糖：花青素 3-O-葡萄糖苷-2"-O-葡萄糖基转移酶，由于 4-bp 插入

DOI：
发表时间：
2005
期刊：
Plant J. 42
影响因子：
0
作者：
Morita Y;Hoshino A;Kikuchi Y;Okuhara H;Ono E;Tanaka Y;Fukui Y;Saito N;Nitasaka E;Noguchi H;Iida S.
通讯作者：
Iida S.

仁田坂英二: "多様なアサガオの変異体と今後の研究展望"農林水産研究ジャーナル. 26. 51-56 (2003)

Eiji Nitazaka：“牵牛花的多样性突变体和未来的研究前景”农林水产研究杂志 26. 51-56 (2003)。