Genetical analysis of sexual behavior in Drosophila melanogasteranalyzes

黑腹果蝇性行为的遗传分析

• 批准号: 07640822
• 负责人: NITASAKA Eiji
• 金额: $ 1.47万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07640822/
• 关键词: Drosophila melnogaster; Mating behavior; Reproductive isolation; P-element; Drosophila simulans; Speciation

To understand the process of speciation, organization of nervous system and mechanism of sex-determination, I have analyzed mating behavior mutations in Drosophila melanogaster. 4000 P-element tagged lines were constructed and sterile or semi-sterile stocks were selected from them.Each sterile strain was tested for its mating behavior, because files with severe mating behavioral defects look like sterile. I obtained several behavior mutation candiates out of 140 sterile strains. Females of the strain #933 have abnormal genitalia and surprisingly they try to court with males but females. More detailed analysis showed that this mutation was a hypomorphic allele of transformer-2. Three mutant stocks with visible phenotype as well as courtship behavior defect in males were identified as new alleles of previously characterized genes. One such mutation was an allele of anterior-open (aop) and this had rough eyes and depigmented tergites. Other two stockes are alleles of scabrous (sca) and rotaed-abdoment (rt). Using males of a D.simulans stock, 37 female mating behavior mutation candidate were obtained from above P-tagged lines. Each mutant showed high repeatability for their mating tests.

为了了解物种形成的过程、神经系统的组织和性别决定的机制，我分析了黑腹果蝇的交配行为突变。构建了4000个P元素标记品系，从中筛选出不育或半不育品系。每个不育品系都进行了交配行为测试，因为具有严重交配行为缺陷的文件看起来像不育。我从140个无菌菌株中获得了几个行为突变候选菌株。933号品系的雌性有异常的生殖器，令人惊讶的是，它们试图与雄性求爱，而不是雌性。更详细的分析表明，该突变是Transform-2的一个亚型等位基因。3个具有明显表型和雄性求偶行为缺陷的突变群体被鉴定为先前特征基因的新等位基因。其中一个这样的突变是前开放(AOP)的等位基因，这种突变有粗糙的眼睛和脱色的老虎。另外两种是粗糙(SCA)和轮腹(RT)等位基因。从上述P标记品系中获得37个雌性交配行为突变候选者。每个突变体在交配试验中都表现出很高的重复性。

项目成果

E.Nitasaka.: "New alleles of eyes color mutations isolated from natural populations in Drosophila melanogaster." Dros.Info.Serv.vol.76. 79 (1995)

E.Nitasaka：“从果蝇自然群体中分离出眼睛颜色突变的新等位基因。”

Nitasaka, E.: "The molecular analysis of brown eye color mutations isolated from geographically discrete populations of Drosphila melanogaster." Molecular and General Genetics. 247. 164-168 (1995)

Nitasaka，E.：“对从地理上离散的黑腹果蝇种群中分离出的棕色眼睛颜色突变进行分子分析。”

Sezutsu, H.: "Evolution of the LINE-like retrotransposon I element in the Drosophila melanogaster species subgroup." Molecular and General Genetics. 249. 168-178 (1995)

Sezutsu, H.：“果蝇物种亚群中 LINE 样逆转录转座子 I 元件的进化。”

B.Richer.: "Nucleotide variation and conservation at the dpp locus,a gene controlling early development in Drosophila." Genetics. (in press). (1997)

B.Richer.：“dpp 位点的核苷酸变异和保守性，dpp 位点是控制果蝇早期发育的基因。”

Nitasaka,E: "Current Topics on Molecular Evolution : Isolation of mating behavior mutations in Drosophila melanogaster" Nei,M.and Takahata,N., 260 (1996)

Nitasaka,E：“分子进化的当前主题：果蝇交配行为突变的分离”Nei,M. 和 Takahata,N.，260 (1996)