Contribution of the Detoxication of Reactive Aldehydes to Protection against Environmental Stress in Plants

活性醛的解毒对保护植物免受环境胁迫的贡献

• 批准号: 15570039
• 负责人: MANO Jun'ichi
• 金额: $ 2.05万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570039/
• 关键词: 2-alkenal reductase; environmental stress; lipid peroxide; aldehyde; phototolerance; chloroplast; acrolein; Calvin cycle; オゾン耐性; α,β-不飽和アルデヒド; alkenal reductase; At5g167970; 抗酸化防御; 活性アルデヒド; 酸化還元酵素

Antioxidative defense in cells is of critical importance in the plant protection against environmental stress.We have recently identified in Arabidopsis thaliana a novel enzyme 2-alkenal reductase (AER) that catalyzes the reduction of the α,β-unsaturated bond of 2-alkenals, potent cytotoxins produced from lipid peroxides. In this study, we aimed at verifying a possible involvement of the lipid peroxide-derived 2-alkenals (reactive aldehydes) in the environmental stress-induced damages of plants. When A.thaliana leaves were oxidatively stressed, production of crotonaldehyde and acrolein was enhanced. Unlike the case for oxidatively stressed animal cells, malondialdehyde was not a major product. Thus, the metabolism of lipid peroxides in plants differs from that in animals. Acrolein and crotonaldehyde inhibited CO_2-fixation in isolated chloroplasts strongly, but did not affect the photosynthetic electron transport activity. Thus the reactive aldehydes specifically inhibit Calvin cycle activity, causing photoinhibition. We constructed three transgenic tobacco lines that over produced the A.thaliana AER.The transgenic plants exhibited higher tolerance to methyl viologen and to intense light, indicating that AER protected leaf cells from oxidative stress. These results altogether indicate the involvement of the reactive aldehydes in the photooxidative injury and the significant contribution of AER to the antioxidative defense in leaves.

细胞内的抗氧化防御在植物抵抗环境胁迫中起着至关重要的作用，我们最近在拟南芥中发现了一种新的2-烯醛还原酶（2-alkenalreductase，AER），该酶催化2-烯醛的α，β-不饱和键的还原，2-烯醛是由脂质过氧化物产生的强细胞毒素。在这项研究中，我们的目的是验证脂质过氧化物衍生的2-烯醛（反应性醛）在环境胁迫诱导的植物损伤的可能参与。氧化胁迫促进了拟南芥叶片中丁烯醛和丙烯醛的产生。与氧化应激动物细胞的情况不同，丙二醛不是主要产物。因此，植物中脂质过氧化物的代谢不同于动物。丙烯醛和巴豆醛强烈抑制离体叶绿体的CO_2固定，但不影响光合电子传递活性。因此，反应性醛特异性地抑制卡尔文循环活性，引起光抑制。我们构建了3个过量表达拟南芥AER的转基因烟草株系，转基因植株表现出对甲基紫精和强光的更高耐受性，表明AER保护了叶片细胞免受氧化胁迫。这些结果表明活性醛参与了光氧化损伤，AER在叶片抗氧化防御中有重要作用。

项目成果

Reactive aldehydes derived from lipid peroxides inhibit the Calvin cycle.

源自脂质过氧化物的反应性醛会抑制卡尔文循环。

• 发表时间: 2006
• 期刊: Plant Cell Physiol. 47
• 作者: Miyatake;F.;Hiraoka;E.;Mano;J.
• 通讯作者: J.

Matsui, K., Sasahara, S., Akakabe, Y., Kajiwara, T.: "Linoleic acid 10-hydroperoxide as an intermediate during formation of 1-octen-3-ol from linoleic acid in Lentinus decadetes"Biosci.Biotechnol.Biochem.. 67. 2280-2282 (2003)

Matsui, K.、Sasahara, S.、Akakabe, Y.、Kajiwara, T.：“亚油酸 10-氢过氧化物作为香菇中亚油酸形成 1-辛烯-3-醇过程中的中间体”Biosci.Biotechnol。

Ascorbate in thylakoid lumen functions as an alternative electron donor to photosystem II and photosystem I

• DOI: 10.1016/j.abb.2004.05.022
• 发表时间: 2004-09-01
• 期刊: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
• 影响因子: 3.9
• 作者: Mano, J;Hideg, É;Asada, K
• 通讯作者: Asada, K

Koeduka, T., Stumpe, M., Matsui, K., 他2名: "Kinetics of barley FA hydroperoxide lyase are modulated by salts and detergents"Lipids. 38. 1167-1172 (2003)

Koeduka, T.、Stumpe, M.、Matsui, K. 和其他 2 人：“大麦 FA 氢过氧化物裂解酶的动力学受盐和去污剂调节”Lipids 38. 1167-1172 (2003)。

Seed deterioration due to high humidity and high temperature is suppressed by extremely low frequency magnetic fields

极低频磁场可抑制因高湿度和高温导致的种子劣化

• 发表时间: 2006
• 期刊: Seed Science ＆ Technology 34・1
• 作者: Jun'ichi Mano;Takeshi Nakahara;Yoshimitsu Torii;Hideki Hirose;Junji Miyakoshi;Koichi Takimoto;Jun'ichi Mano
• 通讯作者: Jun'ichi Mano