Comprehensive analysis on the genes involved in signals for seed formation and seed development

种子形成和种子发育信号相关基因的综合分析

• 批准号: 15570042
• 负责人: SHIMADA Hiroaki
• 金额: $ 2.37万
• 依托单位: Tokyo University of Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570042/
• 关键词: Rice; protein kinase; seed; microarray; endosperm; seed development; クラスター解析

Profiles of expression levels of genes in developing seeds were comprehensively analyzed. The antisense SPK transformant produces watery seeds as the result of abnormal endosperm formation because of lacking accumulation of storage substances. Using immature seeds of this transformant, we analyzed the genes working in the initial stage of seed development. The microarray experiment indicated that 125 genes significantly altered their expression levels in the transformant Among them, several genes showing strong fluctuation were further analyzed to confirm alteration of their expression levels by RT-PCR in detail. In the meantime, microarray analysis was also performed on a mutant which showed the abnormal morphology on the spikes. A large number of gene expression was found to be fluctuated, including genes for SUMO1,F-box proteins, CEN-P proteins. We constructed an antisense transformant on SUMO1 gene and observed its morphology which appeared on the transformant. The transformant resulted in a severe phenotype on the spikelets, suggesting that SUMO is very important on morphogenesis of the spikes.

对种子发育过程中的基因表达谱进行了综合分析。反义SPK转化子由于缺乏贮藏物质的积累而导致胚乳形成异常，从而产生含水种子。利用该转化子的未成熟种子，我们分析了在种子发育的初始阶段起作用的基因。基因芯片实验表明，其中125个基因在转化子中的表达水平发生了显著变化，其中几个基因的表达水平波动较大，通过RT-PCR进一步证实了它们的表达水平发生了变化。同时，还对一个突变体进行了基因芯片分析，发现突变体上出现了异常的形态。发现了大量的基因表达波动，包括SUMO1、F-box蛋白、CEN-P蛋白的基因。我们构建了SUMO1基因的反义转化子，并观察了转化子上的形态。转化子在小穗上表现出严重的表型，表明相扑在小穗的形态发生中起着重要的作用。

项目成果

Very close relationship of the chloroplast genomes among Saccharum species

• DOI: 10.1007/s00122-005-1990-z
• 发表时间: 2005-04
• 期刊: Theoretical and Applied Genetics
• 影响因子: 5.4
• 作者: Sakiko Takahashi;Tsutomu Furukawa;T. Asano;Y. Terajima;Hiroaki Shimada;Akihiro Sugimoto;K. Kadowaki

Complete nucleotide sequence of the sugarcane (Saccharum officinarum) chloroplast genome

甘蔗（Saccharum officinarum）叶绿体基因组的完整核苷酸序列

• 发表时间: 2004
• 期刊: DNA Res. 11
• 作者: Naoki Takahashi;Hirofumi Kuroda;Takashi Kuromori;Takashi Hirayama;Motoaki Seki;Kazuo Shinozaki;Hiroaki Shimada;Minami Matsui;T.Takase et al.;N.Takahashi et al.;T.Asano et al.
• 通讯作者: T.Asano et al.

Molecular characterization of ONAC300,a novel NAC gene specifically expressed at early stage of various developing tissue of rice.

ONAC300的分子特征，一种在水稻各种发育组织早期特异性表达的新型NAC基因。

• 发表时间: 2005
• 期刊: Molecular Genentics and Genomics 272
• 作者: Hiroaki Kusano;Takayuki Asano;Hiroaki Shimada;Koh-ichi Kadowaki
• 通讯作者: Koh-ichi Kadowaki

ydk1-D, an auxin-repsponsive GH3 mutant that is involved in hypocotyl and root elongation.

ydk1-D，一种生长素响应性 GH3 突变体，参与下胚轴和根伸长。

• 发表时间: 2004
• 期刊: Plant Journal. 37
• 作者: Tomoyuki Takase;Miki Nakazawa;Akie Ishikawa;Mika Kawashima;Takanari Ichikawa;Naoki Takahashi;Hiroaki Shimada;Katsuki Manabe;Minami Matsui
• 通讯作者: Minami Matsui

shk1-D, a dwarf Arabidopsis mutant caused by activation of CYP72C1 gene, has altered brassinosteroid levels

shk1-D 是由 CYP72C1 基因激活引起的拟南芥矮化突变体，改变了油菜素类固醇水平

• 发表时间: 2005
• 期刊: Plant Journal 42
• 作者: Naoki Takahashi;Miki Nakazawa;Akie Ishikawa;Kumoko Suzuki;Mika Kawashima;Takanari Ichikawa;Takao Yokota;Hiroaki Shimada;Minami Matsui
• 通讯作者: Minami Matsui