Analysis of a regulation system by a non-coding RNA that is involved in trans-splicing

参与转拼的非编码 RNA 的调控系统分析

• 批准号: 17310115
• 负责人: SHIMADA Hiroaki
• 金额: $ 9.91万
• 依托单位: Tokyo University of Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17310115/
• 关键词: rice; non-coding RNA; gene regulation; transgenic plants; control of translation; upstream ORF; 遺伝子発現調節; 非飜訳RNA; 形質転換体; 飜訳調節; RNAi個体; 発生・再分化; 非コードRNA; アンチセンスRNA; 機能制御; スプライシング; cDNA; 転写

The gene for a protein kinase, SPK, is divided into two parts. The region for the first part, corresponding to the exon 1, is independently expressed and may function as a non-coding RNA. We found a novel gene encoding an unidentified protein, OsMacl, on the opposite strand of the SPK exon 1 region, suggesting that the exon 1 RNA is a natural antisense RNA and may regulate this protein gene. The mRNA for OsMacl contained a long 5' untranslated sequence and there were several small ORFs, upstream ORF (uORF) , in this region. Since OsMacl was predicted to be localized in the cell membrane, this protein was suggested to be function as somewhat involved in external signals. The reported assay using the GUS gene revealed that the GUS gene downstream the uORFs of OsMacl was efficiently translated. These results suggest that there are some unknown regulation system using such uORFs.

蛋白激酶SPK的基因分为两部分。对应于外显子1的第一部分的区域独立地表达，并且可以作为非编码RNA起作用。我们发现一个新的基因编码一个未知的蛋白质，OsMacl，在SPK外显子1区域的相对链上，这表明外显子1 RNA是一种天然的反义RNA，并可能调节该蛋白质基因。OsMac1的mRNA含有一个长的5'端非翻译序列，在该区域有几个小的ORF，即上游ORF（uORF）。由于OsMacl被预测定位于细胞膜中，因此该蛋白被认为在某种程度上参与外部信号的功能。使用GUS基因的报道的测定显示OsMacl的uORF下游的GUS基因被有效地翻译。这些结果表明，有一些未知的调控系统使用这样的uORF。

项目成果

Metabolic engineering of coenzyme Q by modification of isoprenoid side chain in plant

• DOI: 10.1016/j.febslet.2006.01.023
• 发表时间: 2006-02-06
• 期刊: FEBS LETTERS
• 影响因子: 3.5
• 作者: Takahashi, S;Ogiyama, Y;Kadowaki, K
• 通讯作者: Kadowaki, K

uORFを有する機能未知遺伝子の解析

具有 uORF 的功能未知基因的分析

• 发表时间: 2007
• 作者: 寺村 浩;他
• 通讯作者: 他

UORFを有するイネ機能未知遺伝子の解析

具有UORF的水稻功能未知基因分析

• 发表时间: 2007
• 作者: 寺村浩;他
• 通讯作者: 他

Molecular characterization of ONAC300, a novel NAC gene specifically expressed at early stages in various developing tissues of rice

• DOI: 10.1007/s00438-004-1097-2
• 发表时间: 2005-02-01
• 期刊: MOLECULAR GENETICS AND GENOMICS
• 影响因子: 3.1
• 作者: Kusano, H;Asano, T;Kadowaki, K
• 通讯作者: Kadowaki, K