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Molecular mechanism of genetic tumor induction by wound

创伤诱发遗传性肿瘤的分子机制

基本信息

批准号：
07454218
负责人：
SYONO Kunihiko
金额：
$ 4.93万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07454218/
关键词：
genetic tumor wound plant hormone stress Ngro1 genes PR-proteins 遺伝子発現 Nicotiana glranca x N.langsdorffii 奇形腫 PRタンパク質器官分化傷害 PR遺伝子ストレス

项目摘要

Genetic tumors are induced by aging or injury and grow without any plant hormoes in vitro. Since hybrids between Nicotiana glauca and N.langsdorffii (F1) produced typical tumors efficiently, we used them as an experimental materials in this project. Using this material, we have already cloned 17 genetic tumor-specific cDNA (TID clones) by subtraction method. A half of them has a homology to the pathogenesis related proteins and other clones have no homology with any known genes. Ngro1 genes in N.glauca homologous to Agrobacterium rhizogenes have been found to express in genetic tumor specifically.In F1 tissues, by cutting treatments teratomas are formed after dedifferentiation that is followed by continuous cell division. The response which deiffers from the ordinary healing that includes, for example, second cell wall thickening usually observed in plants including the parent plants of F1 pkants, Therefore, we analyzed the way in which each TID transcript responed to stress-related chemicals, such as ABA,methyl-jasmonate and salicylic acid., We also analyzed the responses to plant hormones, an auxin and a cytokinin. The results showed, in general, in F1 tissues transcripts induced by stress-related chemicals were also induced by plant hormones. Ngro1B was found to be induced by auxins. On the other hand, we also obtained three cDNA clones, their transcription was suppressed in genetic tumor cells.

遗传肿瘤是由衰老或损伤引起的，在体外没有任何植物激素的情况下生长。由于灰烟与朗斯多夫烟草(N.langsdorffii，F1)的杂交能够高效地产生典型的肿瘤，因此我们将其作为实验材料。利用该材料，我们已经用消减的方法克隆了17个基因肿瘤特异的cDNA(TID克隆)。其中一半与致病相关蛋白有同源性，其他克隆与任何已知基因没有同源性。与发根农杆菌同源的Ngro1基因已被发现在遗传性肿瘤中特异表达。在F1组织中，通过切割处理，在去分化后形成畸胎瘤，然后进行持续的细胞分裂。不同于普通愈合的反应，包括通常在包括F1植株亲本在内的植物中观察到的第二次细胞壁加厚，因此，我们分析了每个TID转录本对胁迫相关化学物质如ABA、茉莉酸甲酯和水杨酸的反应方式，我们还分析了对植物激素、生长素和细胞分裂素的反应。结果表明，一般来说，在F1组织中，胁迫相关化学物质诱导的转录本也受到植物激素的诱导。Ngro1B被发现由生长素诱导。另一方面，我们还获得了三个克隆，它们在遗传性肿瘤细胞中的转录受到抑制。