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Function of the genes specifically expressed in the genetic tumors

遗传肿瘤中特异性表达的基因的功能

基本信息

批准号：
10440237
负责人：
SYONO Kunihiko
金额：
$ 5.06万
依托单位：
Japan Women's University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10440237/
关键词：
genetic tumor rol Agrobacterium rhizogenes hairy root plant hormone tobacco jasmonic acid transgenic plant 遺伝的腫瘍 rol遺伝子 ACC ORF13 cDNAクローン傷害誘導 Agrobacterium tumefaciens CDNAクローンストレス関連物質

项目摘要

Agrobacterium rhizogenes is responsible for the formation of adventitious roots known as hairy roots. The molecular basis of this disease is the transfer of the DNA segments (Rirol genes) of Ri plasmid into the plant genome. In the genome of untransformed Nicotiana glauca, Ngrol genes, homologous to Rirol genes, were detected. We have already reported NgrolB and NgrolC genes expressed in the genetic tumor tissues spontaneously developed on F1 plants between N. glauca (-group) and N. langsdorffii (+group).In this study, we found NgORF13 also expressed in the genetic tumors. Furthermore, the expression of NgrolB, NgrolC and NgORF13 was found to be regulated by the plant hormones, auxin, cytokinin and jasmonic acid, respectively. On the other hand, the transgenic N. tabacum (-group) revealed both NgORF13 and NgrolC promoted the Rirol-mediated hairy root induction. Overexpression of NgORF13 and NgrolC, under the control of 35S promoter, provoked morphological abnormalities in the transgenic N. tabacum. No tumors, however, developed on these plants.To clarify whether or not the Ngrol genes are the tumor factor in group of tobacco, chimeric genes, such as NgrolB〜ORF14, 35S-NgrolC, 35S-NgORF13, were separately introduced into N. langsdorffii. The tumor formation also couldn't be observed in these cases. The results indicate that Ngrol genes aren't a unique factor of tumor formation in -group. The varied responses to plant growth regulators of these transgenic plants suggests, however, Ngrol genes cooperated with other unknown factor in -group tobacco might function on genetic tumor formation

发根农杆菌负责形成被称为毛状根的不定根。该病害的分子基础是Ri质粒的DNA片段（Rirol基因）转移到植物基因组中。在未转化的烟草基因组中，检测到与Rirol基因同源的Ngrol基因。我们已经报道了NgrolB和NgrolC基因在N. glauca（-group）和N.本研究发现NgORF 13在遗传性肿瘤中也有表达。此外，发现NgrolB、NgrolC和NgORF 13的表达分别受植物激素、生长素、细胞分裂素和茉莉酸的调节。另一方面，转基因N.结果表明，NgORF 13和NgrolC均促进了Rirol介导的毛状根诱导。在35 S启动子控制下，NgORF 13和NgrolC的过表达引起转基因N.烟草为了明确Ngro 1基因是否是烟草组中的肿瘤因子，将嵌合基因如Ngro 1B、35 S-Ngro 1C、35 S-NgORF 13分别导入烟草中。langsdorffii。在这些病例中也不能观察到肿瘤形成。结果表明，Ngrol基因不是群体内肿瘤形成的唯一因素。这些转基因植株对植物生长调节剂的不同反应表明，Ngrol基因可能与烟草中的其他未知因子协同作用，在遗传肿瘤的形成中发挥作用