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Identification of adenohypophysial hormones from the hagfish pituitary gland and its evolutional implication

盲鳗垂体腺垂体激素的鉴定及其进化意义

基本信息

批准号：
15570047
负责人：
NOZAKI Masumi
金额：
$ 2.3万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

The present study aimed to elucidate the pituitary system of hagfish by means of immunohistochemical and molecular biological techniques. Adenohypophysial cell types were studied in the pituitaries of the Atlantic and the Pacific hagfish, using antisera to tetrapod and fish pituitary hormones. Three different types of immunoreactive cells were detected in the adenohypophysis, which appeared to correspond with gonadotropin (GTH)-like, growth hormone (GH)-like and adrenocorticotropin (ACTH)-like cells. In the subsequent study, the relationship between the amount of GTH-like material and gonadal conditions was examined in the brown hagfish (Paramyxine atami), one of the Pacific hagfish. Immunoreactive GTH-like material was heavily accumulated in adults with developing gonads, whereas it was not or only weakly accumulated in juveniles or adults without developing gonads. Following intraperitoneal administration of estradiol benzoate in juvenile animals of P.atami, the intensities of GTH-like material in the adenohypophysis increased significantly when compared to those of controls. cDNAs of both α- and β-subunits of GTH have been cloned from the pituitary gland of P.atami (Nozaki et al., unpublished). The deduced amino acid sequence of the hagfish GTHα shows 39-44% identity to α-subunits of gnathostome glycoproteins, and the hagfish GTHβ shows similar identity (30-43%) with gnathostome LHβ,FSHβ, and TSHβ. These results indicated the presence of GTH in the hagfish pituitary gland.

本研究旨在通过免疫组织化学和分子生物学技术对八脚鳗的脑垂体系统进行研究。用抗四足类和鱼类脑下垂体激素的血清，对大西洋和太平洋鳗的脑垂体细胞类型进行了研究。腺体内有三种不同类型的免疫反应细胞，分别与促性腺激素(GTH)样细胞、生长激素(GH)样细胞和促肾上腺皮质激素(ACTH)样细胞相对应。在随后的研究中，研究了太平洋鳗鱼之一的褐鳗(Paryxine Atami)GTH样物质的数量与性腺状况之间的关系。GTH免疫阳性物质在性腺发育的成人中大量积聚，而在未发育性腺的青少年或成人中未见或仅有少量积聚。腹腔注射苯甲酸雌二醇后，腺垂体内GTH样物质的强度与对照组相比显著增加。GtH的α和β亚基的cDNA都是从红腹鱼的脑下垂体中克隆出来的(Nozaki等人，未发表)。其推导的氨基酸序列与钩吻吻鱼糖蛋白α-亚基的同源性为39-44%，与β的同源性为30-43%，与β、β和β的同源性为30%-43%。这些结果表明GTH存在于鳗鱼脑下垂体中。