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HYPOTHALAMO-HYPOPHYSIAL SYSTEM AND CONTROL OF GONADOTROPIN SECRETION IN THE ELASMOBRANCHS

下丘脑-垂体系统和软骨素促性腺激素分泌的控制

基本信息

批准号：
09640789
负责人：
NOZAKI Masumi
金额：
$ 2.05万
依托单位：
NIIGATA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

Hypothalamo-hypophysial system was studied in some species of elasmobranchs, such as dogfish Scyliorhinus torazame, and common skate Raja porosa, in relation to the control of gonadotropin secretion. The project was focused on 1) distribution of adenohypophysial hormones in the pituitary, 2) distribution of gonadotropin-releasing hormones (GnRHs) in the brain, 3) isolation of gonadotropin from the ventral lobe, and 4) establishment of radioimmuno-assay of dogfish gonadotropin. During the term of this project the research subject was only completed on the distribution of adenohypophysial hormones. The distribution of GnRH-neuronal system was extensively studied using antibodies to dogfish GnRH and other GnRHs. Immunoreactive GnRH-fibers were abundantly observed in the forebrain, but they were few in number in the hypothalamus. Although it is expected that GnRH reaches the ventral lobe via general circulation, it is still unclear from where GnRH is released to the blood circulation. An a … More ttempt to isolate gonadotropin from the ventral lobe was unsuccessful, probably due to high contamination. The following is the abstract of the distribution of adenohypophysial hormones in the dogfish pituitary. ACTH cells and MSH cells stained with anti-porcine ACTH and anti-α-MSH were found in the rostral pars distalis and pars intermedia, respectively. Growth hormone (GH) cells stained with anti-shark GH and anti-sturgeon GH were found in the proximal pars distalis. Somatolactin cells stained with anti-salmon somatolactin were found in the pars intermedia. However, prolactin cells, if present, were not detected by anti-sturgeon prolactin or anti-salmon prolactin. In the ventral lobe, only one type of cells was stained positively by antibodies to glycoprotein hormones or their subunits, with stronger immuno-reaction by anti-sturgeon GTH IIβ, anti-salmon GTH, anti-hCG, and anti-human TSHβ. Since only one type of glycoprotein hormone cell was detected, it was difficult to know whether both GTH and TSH cells are present there. Less

研究了角鲨（角鲨）和棘鱼（鳐）下丘脑-下丘脑系统对促性腺激素分泌的调控作用。本课题主要研究1)垂体腺垂体激素的分布，2)脑内促性腺激素释放激素（GnRHs）的分布，3)腹侧叶促性腺激素的分离，4)角鲨促性腺激素放射免疫检测方法的建立。本课题期间仅完成了腺垂体激素分布的研究课题。使用角鲨GnRH和其他GnRH抗体广泛研究了GnRH神经元系统的分布。免疫反应性gnrh纤维在前脑中大量存在，但在下丘脑中数量较少。虽然预计GnRH通过全身循环到达腹叶，但目前尚不清楚GnRH从何处释放到血液循环。再一次尝试从腹叶中分离促性腺激素都没有成功，可能是由于高污染。以下是角鲨垂体腺垂体激素分布的摘要。抗猪ACTH细胞和抗α-MSH细胞分别在吻侧远部和中部染色。生长激素（GH）细胞染色抗鲨鱼GH和抗鲟鱼GH在近端远部。在pars培养基中发现抗鲑鱼生长肌动素染色的生长肌动素细胞。然而，催乳素细胞，如果存在，没有检测到抗鲟鱼催乳素或抗鲑鱼催乳素。在腹叶，只有一种类型的细胞被糖蛋白激素或其亚基抗体染色阳性，抗鲟鱼GTH IIβ、抗鲑鱼GTH、抗hcg和抗人TSHβ的免疫反应较强。由于只检测到一种糖蛋白激素细胞，因此很难知道那里是否同时存在GTH和TSH细胞。少