权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Characterization of proteins involved in the regulation of sperm motility and their protein network

参与精子活力调节的蛋白质及其蛋白质网络的表征

基本信息

批准号：
15570061
负责人：
INABA Kazuo
金额：
$ 2.37万
依托单位：
University of Tsukuba (2004)Tohoku University (2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

The research was carried out to elucidate the molecular network or cascade from the reception of signaling molecule to the activation of motile machinery in sperm cell. As a result a part of the proteins involved in this signaling pathway have been identified and the molecular interactions among the molecules have been revealed. In the research period twelve proteins that undergo changes in electric point or in the amount were detected in the sperm of the ascidian Ciona intestinalis on two-dimentional gel electrophoresis and peptide mass fingerprinting, Among them, potentially important axonemal proteins could be identified : outer arm dynein intermediate chain2(IC2), an intermediate chain of inner arm dynein termed IC116, radial spoke component LRR37, the regulatory subunit of cAMP-dependent protein kinase and a 94 kDa novel armadillo repeat protein, Another proteins are though to be involved in the reception of signals or intracellular signal transduction, such as SP17-like protein, TER ATPasae,TEFB,and 14-3-3 proteins. On the other hand, protein-protein interaction was examined by means of selective extraction of the axonemes, a series of columm chromatography and immunoprecipitation. As a result, a subcomplex constitution the radial spoke could be isolated. The subcomptex was composed of AKAP-like protein (RSP3), HSP40, two MORN repeat proteins, RSP4/6 and several novel proteins whose homologs are also present in human but the functions have been unrevealed. The molecular interaction in this subcomplex would shed light on the molecular function of radial spoke and ultimately on the mechanism of flagellar motility conserved from ascidian to human. The result also suggests that molecular chaperone is involved in the formation, maintenance or regulation of flagellar motility.

本研究旨在阐明精子细胞从接受信号分子到激活运动机制的分子网络或级联反应。因此，参与该信号通路的部分蛋白质已被鉴定，并且分子之间的分子相互作用已被揭示。在研究期间，通过双向凝胶电泳和肽质量指纹图谱分析，在海鞘Ciona spermatozoa的精子中检测到12种蛋白质，这些蛋白质的电性或量发生了变化，其中可能具有重要意义的轴丝蛋白被鉴定出来：外臂动力蛋白中间链2（IC 2），称为IC 116的内臂动力蛋白的中间链，径向轮辐组件LRR 37，cAMP依赖性蛋白激酶的调节亚基和一个94 kDa的新的犰狳重复序列蛋白。另外一些蛋白质被认为参与信号的接收或细胞内信号的转导，如SP17样蛋白、TERATPasae、TEFB和14-3-3蛋白。另一方面，蛋白质-蛋白质相互作用通过选择性提取的轴丝，一系列的柱层析和免疫沉淀进行了检查。因此，一个子复合体构成的径向辐条可以被隔离。该亚复合体由AKAP样蛋白（RSP 3）、HSP 40、两个MORN重复序列蛋白、RSP 4/6和几个新的蛋白质组成，这些蛋白质在人类中也存在同源物，但其功能尚未被揭示。该复合体中的分子相互作用将揭示辐射辐的分子功能，并最终揭示从海鞘到人类保守的鞭毛运动机制。这一结果还表明，分子伴侣参与鞭毛运动的形成、维持或调节。

项目成果

期刊论文数量（36）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Identification of a novel leucine-rich repeat protein as a component of flagellar radial spoke in the Ascidian Ciona intestinalis.

DOI：
10.1091/mbc.02-06-0089
发表时间：
2003-02
期刊：
Molecular biology of the cell
影响因子：
3.3
作者：
P. Padma;Yuhkoh Satouh;Ken-ichi Wakabayashi;Akiko Hozumi;Y. Ushimaru;R. Kamiya;K. Inaba
通讯作者：
P. Padma;Yuhkoh Satouh;Ken-ichi Wakabayashi;Akiko Hozumi;Y. Ushimaru;R. Kamiya;K. Inaba

Inaba, K.et al.: "Control of flatfish sperm motility by CO_2 and carbonic anhydrase"Cell Motil Cytoskeleton. 55. 174-187 (2003)

Inaba, K.等人：“CO_2 和碳酸酐酶对比目鱼精子活力的控制”Cell Motil 细胞骨架。