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Neural circuit regulating drinking behavior in the eel acclimated to sea water

调节适应海水的鳗鱼饮水行为的神经回路

基本信息

批准号：
15570064
负责人：
ANDO Masaaki
金额：
$ 2.37万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

Although the circumventricular organs(CVOs) in the eel is not identified yet, we identified 3 CVOs in the eel, magnocellular preoptic nucleus (PM), anterior tuberal nucleus (NAT) and area postrema (AP). The PM and the AP were immuno- reactive to vaosotocin (AVT)- and tyrosine hydroxylase (TH)-antibody, respectively (Mukuda et al., 2005).Swallowing is due to relaxation of the upper esophageal sphincter(UES) muscle. After injection of Evans blue into the UES, the glossopharyngeal-vagal motor complex (GVC) was labeled retrogradely, and these neurons were immunoreactive to choline acetyltransfetase-antibody (Mukuda & Ando, 2003). Indeed, the UES muscle (striated muscle) was innervated by a cholinergic neuron and constricted by ACh (Kozaka & Ando, 2003). The vagus nerve was divided to 10 branches (X1-X10) behind the gill arcs. Among these vagal branches, only X5 situated on the internal jugular vain innervated the UES, with optimal frequency of 20 Hz (Ogawa & Ando, in preparation). The existence of optimum frequency seems to be due to Ca^<2+>-ATPase on the sarcoplasmic reticulum (Tamura & Ando, in preparation). On the other hand, the UES was constricted by AVT and relaxed by isotocin via cAMP (Watanabe & Ando, in preparation).Neuronal activity of the GVC was inhibited by catecholamines in a dose-dependent manner, and this inhibitory effects were blocked by yohimbine. Since the vagal lobe (LX), the commissural nucleus of Cajal (NCC), the reticular formation (RF) and the AP in the medulla are immunoreactive to TH-antibody, some of these nuclei may innervate the GVC (Ito, Mukuda & Ando, submitted). Indeed, the GVC activity was inhibited by an electrical stimulation on the LX, but the inhibitory effect was not blocked by yohimbine (Ito & Ando, in preparation). Therefore, it is likely that the GVC activity is regulated by many inputs and the neuronal circuit on the GVC seems to be complicated.

虽然鳗鱼的脑室周围器官(CVO)尚未被识别，但我们在鳗鱼的大细胞视前核(PM)、结节前核(NAT)和最后区(AP)中发现了3种CVO。PM和AP分别对VaVT和TH抗体呈免疫反应(Mukuda et al.，2005)。吞咽是由于食道上括约肌(UES)的松弛。将伊文思蓝注入UES后，舌咽-迷走神经运动复合体(GVC)被逆行标记，这些神经元对胆碱乙酰转移酶抗体呈免疫反应(Mukuda&Ando，2003)。事实上，UES肌(横纹肌)由胆碱能神经元支配，并被ACh收缩(Kozaka&Ando，2003)。迷走神经在鳃弧后分为10支(X1~X10)。在这些迷走神经分支中，仅位于颈内静脉的X5支配UES，其最佳频率为20赫兹(Ogawa&Ando，准备)。最佳频率的存在似乎是由于肌浆网(Tamura&Ando，正在准备中)上的Ca^&lt；2+&gt；-ATPase。另一方面，AVT收缩UES，并通过cAMP(Watanabe&Ando，制剂)使其松弛。儿茶酚胺呈剂量依赖性地抑制GVC的神经元活动，育亨宾可阻断这种抑制作用。由于迷走神经叶(LX)、Cajal连合核(NCC)、网状结构(RF)和延髓AP对TH抗体呈免疫反应，其中一些核团可能支配GVC(Ito，Mukuda&Ando，提交)。事实上，电刺激Lx可抑制GVC的活性，但育亨宾(Ito&Ando，制剂中)并不能阻断这种抑制作用。因此，GVC的活动可能受到多个输入的调节，GVC上的神经元回路似乎很复杂。