Molecular evolutionary study on the phylogeny and body plan of chaetognath.

毛颌动物系统发育和身体规划的分子进化研究。

• 批准号: 15570078
• 负责人: GOTO Taichiro
• 金额: $ 2.3万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570078/
• 关键词: chaetognath; arrow worm; vasa; phylogenetic analysis; ミトコンドリアDNA; 前口動物; PL10

Phylogenetical position of the chaetognaths are always debated. Recent molecular evidence has suggested that they are not deuterostomes but protostomes. Molecular investigations of chaetognath development are much needed to resolve evolutionary relationship. Existence of germ granules is one of the characteristic in chaetognath development. In the present study we examined the vasa-related genes which are involved in germ line in higher metazoans and would be a molecular marker to compare phylogenetic relationship. We cloned the vasa-related gene from the arrow worm Paraspadella gotoi A vasa-related gene (Pgvas1) was isolated and the whole length of Pgvas1 was obtained. The phylogenetic analysis suggested that Pgvas1 was close to a vasa-related gene from the planarian (Plvas1). The result indicates that chaetognath is a very ancient taxon in bilateralians. Pgvas1 was expressed in the PGCs, indicatings the vasa-related gene is involved in germline cells. We have also been trying to isolate homologs of dpp and sog which are involved in the formation of the dorso-ventral axis.

毛颌动物的系统发育地位一直存在争议。最近的分子证据表明它们不是后口动物而是原口动物。为了解决进化关系，非常需要对毛颌发育进行分子研究。胚芽颗粒的存在是毛颌动物发育的特征之一。在本研究中，我们检查了与高等后生动物种系相关的vasa相关基因，并将其作为比较系统发育关系的分子标记。我们从箭虫Paraspadella Gotoi中克隆了vasa相关基因，分离得到了vasa相关基因（Pgvas1），并获得了Pgvas1全长。系统发育分析表明，Pgvas1 与涡虫的 vasa 相关基因 (Plvas1) 接近。结果表明，毛颌动物是双边动物中一个非常古老的分类单元。 Pgvas1 在 PGC 中表达，表明 vasa 相关基因参与生殖系细胞。我们还一直在尝试分离参与背腹轴形成的 dpp 和 sog 的同源物。