Molecular evaluation of volatiles in plant defense

已知有多种类型的挥发物从植物细胞中产生和释放。我们研究了几种不同的挥发物的作用和重要性，其中单萜是柑橘挥发物的主要成分。对11个单萜类化合物进行了杀菌活性、抗虫活性、信号转导活性等生物学活性测试。已知甲戊酸和非甲戊酸途径参与了萜类化合物的生物合成，C10单萜类化合物的合成将由叶绿体中的非甲戊酸途径通过单萜途径由GPP合成。柑橘中至少有7种酶参与了非甲戊酸途径，并克隆了编码其中一种酶2-C-甲基-D-赤霉醇-2，4-环二磷酸合成酶(ISPF)的基因。以GFP为记者的定位分析表明，该基因产物定位于叶绿体中。

Various types of volatiles have been known to produce and release from plant cells. We have studied roles and importance of several different volatiles including monoterpenes, which are the major component of citrus volatiles. Eleven monoterpenes were tested their biological functions, such as fungicidic acitivity, anti-insect activity, and signal transduction activity. Mevalonic acid and non-mevalonic acid pathways have been Known to be involved for biosynthesis of terpenoids and C10 monoterpenes would be synthesized by GPP from non-mevalonic acid pathway in chloroplast through monoterpene pathway. There are at least seven enzymes involved in non-mevalonic acid pathway, and a citrus gene encoding one of these enzymes, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), was cloned. A localization analysis using GFP as the reporter indicated that the gene product is located in chloroplast.