Molecular evaluation of volatiles in plant defense

植物防御中挥发物的分子评价

• 批准号: 15580035
• 负责人: AKIMITSU Kazuya
• 金额: $ 2.11万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580035/
• 关键词: citrus; monoterpene; plant; non-mevalonic acid pathway; signal transduction; Alternaria; ラフレモン; 抵抗性; 揮発性物質; 傷処理; 菌接種

Various types of volatiles have been known to produce and release from plant cells. We have studied roles and importance of several different volatiles including monoterpenes, which are the major component of citrus volatiles. Eleven monoterpenes were tested their biological functions, such as fungicidic acitivity, anti-insect activity, and signal transduction activity. Mevalonic acid and non-mevalonic acid pathways have been Known to be involved for biosynthesis of terpenoids and C10 monoterpenes would be synthesized by GPP from non-mevalonic acid pathway in chloroplast through monoterpene pathway. There are at least seven enzymes involved in non-mevalonic acid pathway, and a citrus gene encoding one of these enzymes, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), was cloned. A localization analysis using GFP as the reporter indicated that the gene product is located in chloroplast.

已知植物细胞会产生和释放各种类型的挥发物。我们研究了几种不同挥发物的作用和重要性，包括单萜，这是柑橘挥发物的主要成分。对11种单萜进行了杀真菌活性、抗虫活性和信号转导活性等生物学功能测试。萜类化合物的生物合成已知有甲羟戊酸途径和非甲羟戊酸途径参与，叶绿体中的非甲羟戊酸途径经单萜烯途径由GPP合成C10单萜烯。在非甲羟戊酸途径中至少有7种酶参与，并克隆了柑橘基因2- c -甲基- d -赤藓糖醇2,4-环二磷酸合成酶（ispF）。利用绿色荧光蛋白作为报告基因进行定位分析，表明该基因产物位于叶绿体中。

项目成果

Biology of Molecular Plant-Microbe Interactions, Volume 4

分子植物-微生物相互作用生物学，第 4 卷

• 发表时间: 2004
• 作者: Ohtani;K.;Masunaka;A.;Yamasaki;Y.;Yamamoto;H.;Akimitsu;K.
• 通讯作者: K.

山崎祐未子, 久能 均, 山本弘幸, 秋光和也: "カンキツ病害抵抗性誘導における揮発性モノテルペンの役割について"日本植物病理学会講演要旨集. 164 (2004)

Yumiko Yamazaki、Hitoshi Kuno、Hiroyuki Yamamoto、Kazuya Akimitsu：“挥发性单萜在诱导柑橘抗病性中的作用”日本植物病理学学会会议记录 164 (2004)。

山崎 祐未子, 田村 啓敏, 久能 均, 山本 弘幸, 秋光 和也: "Alternaria alternataを噴霧接種したラフレモン葉からのモノテルペン放出様式"日本植物病理学会講演要旨集. 12 (2003)

Yumiko Yamazaki、Keitoshi Tamura、Hitoshi Kuno、Hiroyuki Yamamoto、Kazuya Akimitsu：“喷雾接种链格孢菌的粗糙柠檬叶中的单萜释放模式”日本植物病理学学会会议记录 12 (2003)。

Dissection of the host range of the fungal plant pathogen Alternaria alternata by modification of the secondary metabolism

通过修改次生代谢来剖析真菌植物病原体链格孢菌的宿主范围

• 发表时间: 2004
• 期刊: Molecular Microbiology 52(2)
• 作者: Ito;Kaoru
• 通讯作者: Kaoru

Timmer, L.W., Peever, T.L., Dolel, Z., Akimitsu, K.: "Alternaria diseases of citrus - Novel pathosystems."Phytopathologia Mediterranea. 42. 99-112 (2003)

Timmer, L.W.、Peever, T.L.、Dolel, Z.、Akimitsu, K.：“柑橘链格孢病 - 新病理系统。”地中海植物病理学。