Mechanism of disease caused by deficiency of degradation of tRNA intron in mitochondria

编码acr -毒素受体的ACRS基因在柑橘毒素不敏感品种的线粒体中被降解。为了鉴定与ACRS mrna结合蛋白AmBP30组成ACRSmRNA降解蛋白复合物的组成蛋白，以AmBP30为诱饵的酵母两种杂交体，利用AmBP30和y2h选择的蛋白抗体进行免疫沉淀，通过TOF-MS对沉淀蛋白进行分析，确定了多个蛋白为ACRSmRNA降解蛋白复合物的组成蛋白。此外，通过靶基因破坏和RNA沉默，证实了多个基因在ACR和act毒素生物合成中的作用。

ACRS gene encoding ACR-toxin receptor is degraded in mitochondria from the toxin-insensitive citrus cultivars. To identify the component proteins consisting ACRSmRNA degradation protein complex with AmBP30, which is ACRS mRNA-binding protein, yeast two hybrid with AmBP30 as the bait and immuno-precipitation using AmBP30 and Y2H-selected protein antibodies following the precipitated protein analysis by TOF-MS confirmed multiple proteins as the components of ACRSmRNA degradation protein complex. Furthermore, roles of multiple genes in ACR and ACT-toxins biosynthesis were confirmed using target-gene disruption and RNA silencing.