Microbiological evaluation of microbial products for agricultural use

农用微生物产品的微生物学评价

• 批准号: 15580050
• 负责人: SOMEYA Takashi
• 金额: $ 1.92万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580050/
• 关键词: Microbial products; Fluorescence microscopy; Ethidium bromide; CFDA; CTC; FISH; DGGE; Quality control; エチジウムプロミド法; CFDA法; CTC法; 非培養法; エチジウムブロミド法; 生ごみ発酵促進; 堆肥化促進; 環境浄化

To evaluate microbial products commercially available in Japan for agricultural purpose, fluorescence microscopic analyses and PCR-DGGE analysis were applied. Among sixteen microbial products used in dry powder form for agricultural purpose including the enhancement of degradation of raw kitchen garbage and the acceleration of compost process, seven products contained very poor bacteria (10^8 cells/g dry matter or less) estimated by the CFDA vital stain method. Viability (ratio of number of living bacteria by the CFDA method to the number of total bacteria by the ethidium bromide method) was 40% or less and some products showed very poor ratio of even 5% or less. As for liquid microbial products used for various purpose including water treatment, plant growth promotion, etc., poorer results were obtained. Among sixteen liquid microbial products, eight products contained very poor bacteria (10^7 cells/g dry matter or less). Viability was 30% or less and some products showed very poor ratio of even 1% or less. These results suggest that most microbial products used for agricultural purpose in Japan were produced without quality control and microbial viability was very poor.

为了评价日本市售的农业用微生物产品，采用了荧光显微分析和PCR-DGGE分析。在促进厨余生垃圾降解和加速堆肥过程的16种干粉形式农业微生物产品中，CFDA生命染色法估计有7种产品含有极差细菌（10^8个细胞/g干物质）。活性（CFDA法检测到的活菌数与溴化乙啶法检测到的总菌数之比）为40%或更低，有些产品的这一比例很差，甚至不到5%。而用于水处理、促进植物生长等多种用途的液体微生物产品，效果较差。在16种液体微生物产品中，8种产品含有极差细菌（10^7个细胞/g干物质或以下）。存活率为30%或更低，有些产品的存活率甚至只有1%或更低。这些结果表明，日本大部分农业微生物产品的生产缺乏质量控制，微生物活力很差。

项目成果

微生物生態学入門-地球環境を支えるミクロの生物圏-

微生物生态学简介 - 支持全球环境的微生物圈 -

• 发表时间: 2004
• 作者: 染谷 孝(共著)

染谷 孝, 他: "蛍光染色による土壌・堆肥中の特異的微生物検出技術"土と微生物. 57. 115-123 (2003)

Takashi Someya 等人：“使用荧光染色检测土壤和堆肥中特定微生物的技术”《土壤与微生物》57. 115-123 (2003)。

Specificity and Sensitivity of Probes for Fluorescence in situ Hybridization.

荧光原位杂交探针的特异性和灵敏度。

• 发表时间: 2004
• 期刊: Proceedings of the International Symposium on Identification and Use of Microbial Resources for Sustainable Agriculture
• 作者: Wang;X.;T.Sato;K.Inoue;T.Someya
• 通讯作者: T.Someya

染谷 孝: "蛍光染色による土壌微生物の検出法"月間海洋.号外. 33. 14-22 (2003)

Takashi Someya：“通过荧光染色检测土壤微生物的方法”海洋月刊特刊。

Evaluation of microbial products used for compost production by fluorescence microscopy

通过荧光显微镜评估用于堆肥生产的微生物产品

• 发表时间: 2007
• 期刊: Encyclopedia of Agricultural Materials for Soil Amendment(Nosan Gyoson Bunka Kyokai)
• 作者: Takashi Someya