Survey and control of mutagens in a municipal solid waste landfill site

某城市生活垃圾填埋场诱变剂调查与控制

• 批准号: 04650502
• 负责人: SOMEYA Takashi
• 金额: $ 1.28万
• 依托单位: Saga University (1994)University Occupational and Environmental Health, School of Nursing and Medical Technology (1992-1993)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04650502/
• 关键词: Solid waste; Municipal solid waste; Landfill; Leachate; Mutagenicity; Mutagen; Ames test; Environmental micropollutant; エームス法

Ames Salmonella assays were conducted to evaluate the dose of mutagenicity in landfill leachate and municipal solid waste. Laboratory experiments with leachate collected from landfill sites and detailed research on experimental landfill models revealed that increase in leachate mutagenicity during aerobic incubation, which simulated leachate treatment process, was due to the production of mutagens by aerobic metabolism of bacteria. To investigate chemical properties of mutagens in leachate, new Ames strains (TA98 1,8-DNP_6, YG1021, TA98NR,etc.) were used. Tests with nitrate reductase-deficient strains showed the low possibility of the presence of nitrated aromatic hydrocarbons as predominant mutagens. A simple and rapid procedure for concentrating mutagens from leachate was described which involved the use of the Sep-Pak CSP 800 system and the Ames plate mathod. Laboratory experiments showed that the municipal incinerator ash was a good absorbent for mutagens from leachate. A hypothesis on the mechanim of production of mutagens in slid waste landfill sites as well as in leachate treatment facilities was described and the posibble pollution control was discussed.

采用艾姆斯沙门氏菌试验评价垃圾渗滤液和城市生活垃圾的致突变剂量。通过对垃圾填埋场渗滤液的实验室试验和垃圾填埋场模型的详细研究，发现在模拟渗滤液处理过程的好氧培养过程中，渗滤液的致突变性增强是由于细菌的好氧代谢产生了致突变物。为研究垃圾渗滤液中致突变物的化学性质，对新的艾姆斯菌株（TA 98 1，8-DNP_6、YG 1021、TA 98 NR等）进行了筛选。用了硝酸还原酶缺陷菌株的试验表明，硝化芳烃作为主要诱变剂存在的可能性很低。本文介绍了一种用Sep-Pak CSP 800系统和艾姆斯平板法浓缩渗滤液中致突变物的简便、快速方法。实验室试验表明，城市垃圾焚烧炉灰对渗滤液中致突变物有良好的吸附作用。本文对滑坡垃圾填埋场和渗滤液处理设施中致突变物的产生机理提出了假说，并对可能的污染控制进行了讨论。

项目成果

染谷孝: "廃棄物由来の環境汚染物質をバクテリアで監視する" 生物工学. 72. 497-497 (1994)

Takashi Someya：“利用细菌监测源自废物的环境污染物”生物工程 72. 497-497 (1994)。

松藤康司: "埋立地浸出水の変異原活性-浸出水中の有害物質に対する予知指標として-" 都市清掃. 45. 28-35 (1992)

Koji Matsufuji：“垃圾填埋场渗滤液的致突变活性 - 作为渗滤液中有害物质的预测指标 -”《城市清洁》，45. 28-35 (1992)。

Takashi Someya: "Monitoring of environmental micropollutants from solid waste (Jap.)" Seibutsu-kogaku Kaishi. 72 (6). 497 (1994)

Takashi Someya：“监测固体废物中的环境微污染物（日本）”Seibutsu-kogaku Kaishi。

鯉川,寿美子: "廃棄物埋立処分場浸出水に含まれる変異原物質の特性" 破棄物学会論文誌. 3. 51-60 (1992)

Koikawa, Sumiko：“垃圾填埋场渗滤液中所含诱变剂的特征”，日本废弃物学会杂志，3. 51-60 (1992)。

大村,実: "廃棄物最終処分場の浸出水に含まれる変異原物質の濃縮方法の比較検討" 水環境学会誌. 15. 52-61 (1992)

Minoru Omura：“最终废物处理场渗滤液中所含诱变剂浓缩方法的比较研究”《水环境学会杂志》15. 52-61 (1992)。