Characterization of humic substances aimed at using for the removal of pollutants

腐殖物质的亲水性与其与重金属的络合作用有关，而疏水性与其对多环芳烃的吸附作用有关。为了表征基于其亲水性和疏水性的腐殖物质，反相高效液相色谱法（RP-HPLC）应用于腐殖酸和富里酸。负责与重金属络合和多环芳烃的吸附的馏分估计通过RP-HPLC与荧光检测。在此基础上，研究了腐殖酸对植物吸收重金属的影响：1.采用C18聚合物柱，采用pH梯度洗脱和乙腈浓度梯度洗脱，将腐殖酸按亲水性和疏水性分为3个组分; 2.采用聚合物整体柱，提高分离能力，腐殖酸和富里酸被分离成五种组分， 关于我们 级分（I-V）。级分I是最亲水的，而级分V最疏水。富里酸的特征是组分I占优势。腐殖酸的组成随腐殖化程度的不同而不同。利用各组分峰面积进行主成分分析，结果表明，以组分V的峰面积区分胡敏酸和富里酸，以组分II-3的峰面积区分A型胡敏酸和其它类型胡敏酸。IV. 3.根据荧光猝灭估计，负责与重金属络合的级分是与重金属络合的级分I和级分II。腐殖酸和仅级分I为富里酸。根据芘与腐殖酸反应时荧光强度的增加，认为对多环芳烃吸附起作用的组分为腐殖酸的全部组分和富里酸的II ~ IV组分。4.施用堆肥腐殖酸增加了Cu在土壤中的溶解度，从而增加了印度芥菜对Cu的吸收。少

The hydrophilic property of humic substances is related to the complexation with heavy metals, whereas the hydrophobic property with the adsorption of polycyclic aromatic hydrocarbons (PAHs). To characterize humic substances based on their hydrophilicity and hydrophobicity, reverse-phased high performance liquid chromatography (RP-HPLC) was applied to humic and fulvic acids. The fractions responsible for the complexation with heavy metals and for the adsorption of PAHs were estimated by RP-HPLC with fluorescence detection. Furthermore, the effects of humic substances on the absorption of heavy metals by plant were investigated.1.When pH gradient elution and acetonitrile concentration gradient elution were applied to RP-HPLC using a C18 polymer column, humic acids were separated into three fractions according to their hydorphilicity and hydrophobicity.2.When used a polymer monolith column was used for RP-HPLC to increase resolving power, humic and fulvic acids were separated into five f … More ractions (I-V). The fraction I was most hydrophilic, whereas the fraction V most hydrophobic. Fulvic acids were characterized by a dominance of fraction I. For humic acids, however, the composition varied with the degree of humification. The principal component analysis using the peak area of each fraction showed that humic acid and fulvic acid were discriminated by the peak area of fraction V and the A type humic acids were discriminated from the other types of humic acids based on the peak areas of fractions II-IV.3.It was estimated from the quenching of fluorescence that the fractions responsible for the complexation with heavy metals were the fractions I and II for humic acids and only the fraction I for fulvic acids. On the other hand, the fractions responsible for the adsorption of PAHs were considered to be the all the fractions for humic acids and the fractions II to IV for fulvic acids based on the increase in the fluorescence when pyrene was react with the humic substances.4.The application of compost-derived humic substances increased the solubility of Cu in soil, and thus increased the absorption of Cu by Indian mustard (Brassica juncea). Less