Study on the structure elucidation and their activity of impulse generating substances from Albizzia julibrissin

合欢冲动产生物质的结构解析及其活性研究

• 批准号: 15580092
• 负责人: NAKAJIMA Shuhei
• 金额: $ 1.34万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580092/
• 关键词: Electrophysiology; Mimosa pudica L.; Albizzia julibrissin; Electrical pulses; Impulse generating substance; Cucumis sativus; Arabidopsis thaliana; Stomatal aperture test; 植物活性電位; マメ科植物; 植物活動電位; スクリーニング

Although much attention has been paid on the leaf movement of Mimosa pudica with irritation and the mechanism for the rapid conduction of stimulus is still controversial in the plant, we found that M.pudica was very sensitve to the administration of extraneous substances and such stimulation afforded electrophysiological responses. Actually, during observation of resting potential strong pulses were recorded by an administration of methanol extract of leguminous plants. We then searched for the substance responsible for the activity in Albizzia julibrissin. In this study, a few miligram of the relatively unsable compound was isolated as an active principle from the hexane soluble fraction of crude methanolic extract of A.julibrissin (2 kg) in 2003. From the other extract of this plant (5〜6 kg), more forty miligram of the active compound was also obtained in 2004. Spectral analysis of the active compound led us to have their partial structures, but the whole structure still remained to be determined. Electrophysiological responses were observed when the active compound was administered to the other plant species, such as Cucumis sativus. We investigated the effect of this compound on the stomatal aperture of Arabidopsis thaliana and we found that this compound also inhibited the germination of lettuce seed.

虽然含羞草叶片在刺激下的运动受到了广泛的关注，刺激在植物中的快速传导机制仍存在争议，但我们发现含羞草对外界物质的刺激非常敏感，这种刺激产生了电生理反应。实际上，在观察静息电位的过程中，通过施用豆科植物的甲醇提取物记录了强脉冲。然后，我们寻找负责合欢中活性的物质。在这项研究中，几毫克的相对不稳定的化合物作为活性成分从2003年的A.julibrissin的粗甲醇提取物（2 kg）的己烷可溶性级分中分离出来。2004年，从该植物的其他提取物（5 - 6 kg）中也获得了40毫克以上的活性化合物。活性化合物的光谱分析使我们得到了它们的部分结构，但整个结构仍有待确定。当将活性化合物施用于其他植物物种如黄瓜时，观察到电生理学反应。我们研究了该化合物对拟南芥气孔开度的影响，发现该化合物还抑制莴苣种子的萌发。