Search for biologically active substances of plants by using the electrophysiological method with excitatory plant cells of Mimosa pudica

利用含羞草兴奋性植物细胞电生理学方法寻找植物生物活性物质

• 批准号: 07806016
• 负责人: NAKAJIMA Shuhei
• 金额: $ 0.51万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07806016/
• 关键词: Electrophysiology; Mimosa pudica L.; Melia azedarach L.; Albizzia julibrissin Durazz; Sodium-glutamate; Screening; 5-hydroxypipecolic acid; 環状アミノ酸

Although much attention has been paid on the leaf movement of Mimosa pudica with irritation and the mechanism for the rapid conduction of stimulus is still controversial in the plant, we found that M.pudica L.was very sensitve to the administration of extraneous substnces and such stimulation afforded electrophysiological responses. Actually, during observation of resting potential strong pulses wre recorded by an admistration of methanol extract of other plants. We then searched for the substance responsible for the activity in Melia azedarach L.as an example. ^<13>C- and ^1H-NMR,FT-IR and mass spectrometric analyzes, as well as alkaline metal identification by a flame analysis demonstrated that the isolated active compound was sodium glutamate. Although commercial L-sodium glutamate showed the same strong pulses in the identical assay, unnatural sodium D-glutamate did not afford such pulse at all. This unambiguously suggests that the interaction of the amino acid with the plant tissue was strictly diastereoselective at the molecular level. From Albizzia julibrissin, two compounds were isolated as active principles, one of which was identified as 5-hydroxypipecolic acid and other's structure still remains to be determined. These studies provide the first example to suggest that measuring the electric pulses produced by M.pudica could be utilized as a method for screening biologically important compounds in plant kingdom.

尽管含有刺激的含羞草叶片运动受到了广泛关注，而且植物中刺激快速传导的机制仍然存在争议，但我们发现含羞草对外来物质的施用非常敏感，并且这种刺激会产生电生理反应。实际上，在观察静息电位时，通过施用其他植物的甲醇提取物记录了强脉冲。然后，我们以苦楝为例，寻找负责该活性的物质。 13 C-和1 H-NMR、FT-IR和质谱分析以及通过火焰分析的碱金属鉴定证明分离的活性化合物是谷氨酸钠。尽管商业 L-谷氨酸钠在相同的测定中显示出相同的强脉冲，但非天然 D-谷氨酸钠根本不提供这样的脉冲。这明确表明氨基酸与植物组织的相互作用在分子水平上是严格非对映选择性的。从合欢中分离得到两种活性成分，其中一种被鉴定为5-羟基哌啶酸，另一种的结构仍有待确定。这些研究提供了第一个例子，表明测量 M.pudica 产生的电脉冲可以用作筛选植物界中具有重要生物学意义的化合物的方法。

项目成果

Shuhei Nakajima et al.: "Electrical pulse generation from Mimosa pudica by administering other plant extracts." Bioscience,Biotechnology,and Biochemistry. 57(11). 1976-1977 (1993)

Shuhei Nakajima 等人：“通过施用其他植物提取物从含羞草中产生电脉冲。”

Shuhei NAKAJIMA et al.: "Electrical Pulse Generation from Mimosa pudica by Administering Other Plant Extracts." Bioscience,Biotechnology,and Biochemistry. 57. 1976-1977 (1993)

Shuhei NAKAJIMA 等人：“通过施用其他植物提取物从含羞草中产生电脉冲。”