Inpulse generating substances of leguminous plants by using the electrophysiological method with excitatory plant cells of Mimosa pudica

含羞草兴奋性植物细胞电生理法研究豆科植物脉冲产生物质

• 批准号: 10660113
• 负责人: NAKAJIMA Shuhei
• 金额: $ 1.02万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660113/
• 关键词: Electrophysiology; Mimosa pudica L.; Albizzia julibrissin; Leucaena glauca; Electrical pulses; Screening; 5-hydroxypipecolic acid; 生物電気; 植物活動電位; 環状アミノ酸

Although much attention has been paid on the leaf movement of Mimosa pudica with irritation and the mechanism for the rapid conduction of stimulus is still controversial in the plant, we found that M. pudica L. was very sensitive to the administration of extraneous substances and such stimulation afforded electrophysiological responses. Actually, during observation of resting potential strong pulses were recorded by an administration of methanol extract of some leguminous plants. We then searched for the substance responsible for the activity in Albizzia julibrissin and Leucaena glauca. In the previous study, from A. Julibrissin, two compounds were isolated as active principles, one of which (active compound 1) was identified as 5-hydroxypipecolic acid. ィイD113ィエD1C- and ィイD11ィエD1H-NMR, FT-IR, UV and mass spectrometric analyses demonstrated that the active compound 2 had a molecular formula (CィイD228ィエD2HィイD250ィエD2NィイD22ィエD2OィイD23ィエD2) and functional groups such as methyl, ethyl, isopropyl, conjugated ketone and methyl ketone in the molecule. However, due to the unstability of active compound 2, the chemical structure still remains to be determined. On the other hand, from the methanol extract of leaves of L. glauca., active compound 3 was isolated and its structure and activity was investigated. These studies provide the suggestion that measuring the electrical pulses produced by M. pudica could be utilized as a method for screening biologically important compounds in plant kingdom.

虽然含羞草叶片在刺激下的运动受到了广泛的关注，刺激在植物体内的快速传导机制仍存在争议，但我们发现M. [医]布地卡属对外来物质的给药非常敏感，并且这种刺激提供电生理学反应。实际上，在观察静息电位的过程中，记录了一些豆科植物的甲醇提取物的管理强脉冲。然后，我们寻找负责合欢和银合欢中的活性的物质。在先前的研究中，从A. Julibrissin，分离出两种化合物作为活性成分，其中一种（活性化合物1）被鉴定为5-羟基哌啶酸。通过对活性化合物D113的D1C-和D11的D1 H-NMR、FT-IR、UV和质谱分析，证实了活性化合物2具有分子式（C D228 D2 H D250 D2 N D22 D2 O D23 D2），分子中具有甲基、乙基、异丙基、共轭酮和甲基酮等官能团。然而，由于活性化合物2的不稳定性，其化学结构仍有待确定。另一方面，从L. glauca.，分离出活性化合物3，并对其结构和活性进行了研究。这些研究表明，测量M. Pudica可作为筛选植物界重要生物活性化合物的一种方法。