Inpulse generating substances of leguminous plants by using the electrophysiological method with excitatory plant cells of Mimosa pudica

对Mimosa pudica的叶子运动进行了大量的关注，并对刺激的快速行为的机制进行了讽刺，我们在植物中找到了M.。pudica L.它对外部物质的管理和这种刺激有影响的电磁学反应非常敏感。Actually， during Observation of resting potential strong pulses were recorded by an administration of methanol extract of some leguminous plants。然后，我们在Albizzia Julibrissin和Leucaena glauca寻找对活动的物质责任。在先前的研究中，来自A。Julibrissin有两种化合物作为活性原则被隔离，其中一种（活性化合物1）被识别为5-羟基环氧树脂。D113 D1C-and D11 D1H-NMR， FT-IR， UV and mass spectrometric analyses demonstrated that the active compound 2 had a molecular formula （C228 YD2H D250 YD2N YD2O YD23 YD2） and functional groups such as methyl， ethyl， isopropyl， conjugated ketone and methyl ketone in the molecule。However，由于活性化合物的不稳定性2，化学结构仍有待确定。在另一边，从L的叶子中提取出甲醇。Glauca，活动化合物3是孤立的，其结构和活动是调查的。这些研究提出了一项建议，用于衡量M.所产生的电脉冲。Pudica可以被用作在植物王国中筛选生物学上重要的化合物的方法。

Although much attention has been paid on the leaf movement of Mimosa pudica with irritation and the mechanism for the rapid conduction of stimulus is still controversial in the plant, we found that M. pudica L. was very sensitive to the administration of extraneous substances and such stimulation afforded electrophysiological responses. Actually, during observation of resting potential strong pulses were recorded by an administration of methanol extract of some leguminous plants. We then searched for the substance responsible for the activity in Albizzia julibrissin and Leucaena glauca. In the previous study, from A. Julibrissin, two compounds were isolated as active principles, one of which (active compound 1) was identified as 5-hydroxypipecolic acid. ィイD113ィエD1C- and ィイD11ィエD1H-NMR, FT-IR, UV and mass spectrometric analyses demonstrated that the active compound 2 had a molecular formula (CィイD228ィエD2HィイD250ィエD2NィイD22ィエD2OィイD23ィエD2) and functional groups such as methyl, ethyl, isopropyl, conjugated ketone and methyl ketone in the molecule. However, due to the unstability of active compound 2, the chemical structure still remains to be determined. On the other hand, from the methanol extract of leaves of L. glauca., active compound 3 was isolated and its structure and activity was investigated. These studies provide the suggestion that measuring the electrical pulses produced by M. pudica could be utilized as a method for screening biologically important compounds in plant kingdom.